Study On The Interaction Between Nsaids Drugs And Biomacromolecule By Fluorescent Spectrometry

The study of the interaction between small drug molecules and biomacromolecule is valuable for the fields of pharmacokinetic,biochemistry,pharmacy and clinical medicine. fluorescent spectrometry offers extending good advantages in terms of high sensitivity, selectivity, convinence and accuracy, then it is widely used in trace-elemental analysis fields in recent years. The effect of metal ions on the interaction between drugs and biomacromolecule was investigated by means of UV and fluorescence spectroscopy. The binding mechanism,constant K andΔH,ΔS,ΔG of drugs to biomacromolecule were obtained.So understanding the interaction mechanism between drugs and biomacro-molecule has an important significance in illustrating transport and metabolize mechanism of drugs into the organism, toxicity and giving the relationship between the structure and the function of protein. Research on the interaction between quinolones,tetracyclinestcs and biomacro-molecule has been conducted by a lot of scholars,but the research on the interaction between nsaids drugs and biomacromolecule has conducted seldomly by scholars.The interaction of nsaids drugs with biomacromolecule was studied by UV/Vis and fluorescence spectroscopy method。The studies were performed three aspects as such follow:Part 1 Micellar Sensitization Spectrophotometric Determination of nsaids drugs.Determination of flurbiprofen by Micellar Electrokinetic Chro-matography has been studied by scholar,a novel method for the determination of flurbiprofen by means of micellar sensitization spectrophotometric detection was proposed. We studied the fluorescence spectra of the system and optimized the experiment conditions.Following the optimally version designed according to the method,we determined limit is low,the recovery is within the range of 99.2%-100.4%, and the relative standard deviation is 1.8%.The results are satisfied.Part 2 Studied the interaction of nsaids drugs and biomacromolecule binary complexes.The interaction of Flurbiprofen and DNA in physiological condition was investigated by fluorescence and UV absorption spectrophotometry. The experiment demonstrated that there were groove binding interaction mode between flurbiprofen and DNA by experiment of the effect of ionic strength, change of UV absorption spectrum and the effect of I" quenching. The results show that the interaction of flurbiprofen and DNA is fluores-cence quenching process.The binding constant is 5.53×105L·mol-1,and the binding site is 1.12.Part 3 The influences of nsaids (meloxicam,fenbufen,indom etacin) on the fluorescence of bovine serum albumin(BSA), metallic ions (zinc (Ⅱ),copper(Ⅱ)) on that of bovine serum albumin,and metallic ions on the of nsaids and bovine serum albumin were studied under imitated the physiological condition.It was shown that both nsaids and metallic ions have a powerful ability to quench the BSA fluorescence via a nonradiative energy transfer mechanism.But the fluorescence quenching action of nsaids on BSA was much stronger in the presence of metallic ions.The fluorescence quenching data were analyzed according to doublereciprocal equation,and the binding constant(K) and the binding sites(n) were obtained,according to the thermodynamic parameters, the main acting force among them was determined.