| BackgroundSelenium which is a necessary micronutrient for human and animal life and activities, plays an important biological effects, such as antioxidant, enhance immunity, antitumor, detoxification for toxic heavy metals, and so no. The deficiency of Se would result in Keshan disease, Kashin-Beck disease and other various of diseases. The data of Chinese Nutrition Society surveyed show:the intake of dietary Se of Chinese Inhabitant is generally more lower, therefore, it is very necessary to supplement Se by the kind of selenium fortification. It have been relatively full about the researches of the acute and subacute toxicological aspects and the physiological function for all kinds of selenium fortification, but it is short of the data of horizontal comparison about the assimilate and bioavailability for selenium fortification.ObjectivesThis study was designed to compare horizontally for Sodium Selenite, Selenium-Enriched Yeast and L-Se-Methylselenocysteine by establishing the model of low-Se rats and then givening the rats of different type and different doses of selenium fortification. The objective is to provide scientific guidance of reinforce Se for the low Se group and to provide a scientific basis for the selenium fortified in food by comparing the effect of assimilate and bioavailability in different doses of SS, SY and L-SeMC for low-Se rats.MethodsWeaning, SPF, male SD rats 104 were, set up the model of low-Se rats. These rats were randomly divided into 13 groups based on their weight, one is control group, SS, SY, and L-SeMC are each four groups. The control group was given pure water everyday, and the other groups were given SS, SY, and L-SeMC respectively according to the dose of 2,4,8,16μg/kg bw for 4 weeks. After 24 hours given the last selenium fortification, the rats were killed to sample the blood and their liver and kidney tissue, then the blood were separated into serum, the liver and kidney tissue were made homogenate. The Se-contents in serum, liver and kidney, the Glutathione Peroxidase activities in serum, liver and kidney and the Thioredoxin Reductase activities in liver and kidney were measured.ResultsThe Se-contents in serum, liver and kidney of rats supplemented by SS, SY and L-SeMC were significantly higher than control group in the Se-complementary range of 2～8μg/kg bw (P＜0.001, P＜0.01, P＜0.05). And The Se-contents in serum, liver and kidney were rosed with the dose increased of supplemented Se (P＜0.001). In rising the Se-contents in serum, liver and kidney, the effect of SY and L-SeMC were better than SS at the same level of supplemented Se (P＜0.001, P＜0.05). The GSH-Px activities in serum, liver and kidney of each dose group of SS, SY and L-SeMC were markedly different with control group (P＜0.001, P＜0.01). In rising the GSH-Px activities, the effect of SY and L-SeMC were better than SS at the same level of supplemented Se (P＜0.001). When the dose of supplemented Se was 16μg/kg bw, the TrxR activities of SS was markedly higher than the control group (P＜0.001). The TrxR activities in liver and kidney of each dose group of SY and L-SeMC were different with control group (P＜0.001, P＜0.01, P＜0.05). At the same level of supplemented Se, the effect of SY and L-SeMC were both better than SS (P＜0.001,P＜0.05).ConclusionSS, SY and L-SeMC were all significantly raised the Se-contents in serum, liver and kidney and the GSH-Px activities in serum, liver and kidney. And both SY and L-SeMC two organic selenium can significantly strengthened the TrxR activities. The effect of raising the Se-contents and enhancing the GSH-Px activities in serum, liver and kidney for SY and L-SeMC were both better than inorganic selenium SS. The assimilate and bioavailability of organic selenium were more better for the rats.The effect of raising the Se-contents in serum for SY was better than L-SeMC; but the effect of enhancing the two antioxidant enzyme activities in the organization for L-SeMC were better than SY.
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