Role Of Caveolin-1 In Differentiation Of Rat Bone Marrow Mesenchymal Stem Cells Into Neurons

AIMMSCs are transfected with small intering RNA (siRNA).To investigate the role of Caveolin-lin bone marrow mesenchymal stem cells(MSCs) differentiating into neurons.MOTHEDSThe experiments were divided into non-transfected group,tranfected group(tranfected with Rn-caveolin-1 siRNA),positive control group(transfected with Rn-MAPK-1 control siRNA) and negative control group(transfected with Negative control siRNA).construct caveolin-1 siRNA and then transfect into MSCs cultured in vitro.MSCs was induced to differentiate into neurons byβ-ME.The fluorescence expressed by transfected MSCs were observed with inverted fluorescence microscope.The expression of caveolin-1 and MAPK-1mRNA was detected by RT-PCR.The neural cell specific marker NSE,NF-M,GFAP were detected by immunocytochemistry stain technique.The survival ratio of MSCs was detected by MTT.RESULTS1.The fluorescence of MSCs was mostly displayed after tranfection of 72 hour and the efficiency of transfection was up to (81.5±2.8)%; Meanwhile,the caveolin-1mRNA expressed by MSCs of transfected group was decreased (P＜0.05); MTT displayed that the survival ratio of MSCs of transfected group hadn't significant difference among groups.2.β-ME induced MSCs into neural cell.The differetiation efficiency of MSCs transfected had the best effect and the expression of Nestin,NF-M were increased significantly comparing with the other groups (P＜0.01)3.The expression of caveolin-1 were increased persistently correspenting with time and There are the highest expression after 6d of induction,besides,There was significant difference between before induction and 6d after induction.CONCLUSIONThe differentiation efficiency of MSCs increased by suppressing Caveolin-1 expression.lipid rafts,which Caveolin-lis marking protein could have important role in regulating MSCs differentiate into neurons.