Comparative Study Of Ability Of Mesenchymal Stem Cell From Bone Marrow And Adipose Tissue Proliferated And Differentiated Into Neurons

Objective:To compare the ability of bone marrow mesenchymal stem cells(BMSCs) and adipose-derived mesenchymal stem cells(ADSCs) from same SD rats proliferated and differentiated into neurons. Methods:The BMSCs and ADSCs from the same SD rats was separated and cultured in vitro, and morphology and proliferation rate of cells was observed and compared. Furthermore, BMSCs and ADMSs was induced and differentiated into neurospheres and final neurons, then the differences of induction and differentiation was compared by immunofluorescence and the expression of neurotrophic factor BDNF and NT-3was detected by ELISA. Results:There is a slight difference in cell morphology between BMSCs and ADMSs, but proliferation of ADSCs is faster than that of BMSCs which is shown on the growth curve.Both BMSCs and ADSCs are easily to be induced into neural stem cells and terminal nerve cells by using fractional step method,the rate of ADSCs and BMSCs induced into neural stem cells was21.73±3.46%and15.62±2.37%,respectively. the rate of ADSCs is higher and faster than those of BMSCs.The results of immunofluorescence staining showed that ADSCs present a higher positive rate of (3-tubulin III than that of BMSCs, which is22.18±8.91%and17.98±11.43%, respectively. In addition, ADSCs can secrete much more BDNF and NT-3than those of BMSCs before and after induction that present at ELISA, the differences were statistically significant(P<0.05). Conclusion:Compared with BMSCs, ADSCs is easy to be obtained and secrete much more neurotrophic factors. The ability of proliferation and rates of neural differentiation of ADSCs is faster and higher than those of BMSCs. ADSCs is a good alternative source of BMSCs in the stem cell transplant for the treatment of central nervous system diseases.