| PurposeForecast combined detection of hsCRP, RBP4 can be used as to determine a more effective disease detection and prognosis of the stability index. Made with economic and social.We evaluated associations of serum retinol-binding protein 4 (RBP4) high-sensitivity C-reactive protein (hsCRP) with stable cardiovascular disease (CVD) and prognosis and selctive coronary angiography. Whether combined detection hsCRP, RBP4 dose of serum could be a more effective way to forcast the stability situation and prognosis of cardiovascular disease (CVD). With the rapid developement of economic and social, people lifestyle, eating habits change, coronary heart disease incidence and morbidity, mortality rates rapidly increased, coronary heart disease on human health pose a huge threat, the vast majority of the underlying coronary heart disease causes of coronary artery atherosclerosis (AS). Atherosclerosis (AS) is a chronic inflammatory proliferative disease, inflammation occurs in the progress of AS in all stages play an important Role. There are many and deep researchs on hsCRP with coronary heart disease;and researchs on RBP4 with coronary heart disease are turning more and more at home and abroad, but resarchs on RBP4and hsCRP with coronary heart disease and coronary artery disease are rare. In this study,85 cases by the subjects were divided into control group of patients at the standard 21 patients with stable angina pectoris (SAP) group,12 cases of unstable angina pectoris (UAP) 42 cases of acute myocardial infarction (AMI) group of 10 cases. Including unstable angina pectoris (UAP) is further divided into single-vessel disease group (UAP-1) 16 cases, double-vessel disease group (UAP-2) 13 cases,3 lesions (UAP-3) After admission of patients in each group were taken at the hospital within 24h fasting 3ml (AMI and UAP patients taken immediately after admission) into lithium heparin tube, shake,3000r/min,10 min, separation of serum and to take home the top serum 0.5ml in the PPV tube-80℃to save under test, RBP4 concentrations of applications for the investigation. High-sensitivity C-reactive protein in each group were taken at the hospital within 24h fasting 3ml (AMI and UAP patients taken immediately after admission), with normal sterile tube without anticoagulant, at room temperature (20～25 degrees Celsius), to be solidified after the extraction placed in the upper PPV serum 0.5ml tube,-80℃to save under test. All patients have never catched rheumatism, rheumatoid diseases,2-type diabete, acute and chronic inflammatory diseases, cancer, infectious diseases, autoimmune diseases, severe obesity, underweight, serious liver and kidney dysfunction, blood diseases, heart disease, valvular disease, and there are not clear cerebral vascular or peripheral vascular disease patients. All patients'diagnoses are tested by clinical and biochemical, ECG and selectively coronary angiography Serum RBP4 concentrations are detected by double antibody ABC-ELISA method; serum CRP concentrations are detected by using immunofluorescence assay for quantitative detection; Selective coronary angiography ofen uses Judikin's catheter technology, most of them through the right radial channels artery, a few through the right femoral artery or the left radial channels artery, conventional multi-position projection.. Two or more experienced physician determine the stenosis level of coronery arteary by ilmaging results.Coronary heart disease diagnostic criteria for coronary angiography is at least one blood vessel diameter stenosis greater than 50%,including left anterior descending artery, left circumflex and right coronary artery; left main artery involvement more than 50%is counted as double-vessel, left main and left anterior descending artery, left circumflex branch do not cumulate; coronary angiography coronary artery diameter stenosis less than 50%is named control group.All quantitative data analysis used SPSS13.0 statistical packages. Measurement data with (?)±sd representation; more than a few between the two groups were compared using single factor analysis of variance, LSD test was used to compare between the two groups; count data x2 test was used to compare between the two groups. P<0.05 for significant difference, P<0.01 for the difference was significant.Results(1) The four groups of serum retinol-binding protein 4 concentrationsSerum RBP4 concentrations of unstable angina pectoris group (49.09±6.60ng/ml), acute myocardial infarction group (51.37±10.09ng/ml) are significantly higher (43.10±8.79ng/ml) and stable angina pectoris group (43.44±9.54ng/ml), there is significant statistical difference (P<0.05). Unstable angina pectoris and acute myocardial infarction between the two groups had no statistical significance (P>0.05), stable angina pectoris group and control group, no obvious statistical significance (P >0.05)(2) Serum high-sensitivity C-reactive protein concentrationThe serum concentration of high-sensitivity C-reactive protein with unstable angina pectoris group (4.08±1.95mg/l), acute myocardial infarction group (4.84±1.72mg/l) are significantly higher than that of stable angina pectoris group (1.51±0.73mg/l)and control group(1.89±0.42mg/l), there is a significant statistical difference(P<0.01). The hsCRP serum concentrations of unstable angina pectoris and acute myocardial infarction are different,but there isn't statistical significance difference (P>0.05), there are not statistical significance difference in serum hsCRP concentrations between the stable angina pectoris and the control group (P>0.05)(3) (Coronary heart disease) Serum hsCRP concentration and coronary angiography in high-sensitivity C-reactive protein serum levels than the control group,21 cases (1.51±0.73mg/1) was significantly lower than single-vessel disease, unstable angina 28 patients (3.86±2.27mg/l),2 lesions were 26 cases (4.13±1.86mg/l),10 patients with triple vessel disease group (3.64±1.27mg/l) concentration in the control group compared with the lesion group were significantly different (P<0.01): single, double vessel, the concentration difference between the three disease groups, no statistical significance (P>0.05).(4) (Coronary heart disease) Serum RBP4 concentration and coronary angiography in retinol binding protein 4 concentration in the control group compared 21 cases (43.10±8.79ng/ml) single-vessel disease,28 cases (49.18±7.32ng/ml),2 lesions were 26 cases (50.10±5.77ng/ml), triple vessel disease 10 cases (51.73±6.65ng/ml) difference was statistically significant (P<0.05); single, double sticks, three vessel disease, the concentration difference between groups, no statistical significance (P>0.05).Conclusion1.The serum concentration of high-sensitivity C-reactive protein and retinol binding protein 4 in both UAP and AMI group were significantly higher than that of SAP group and control group; Based on these results we suppose that the serum concentration of high-sensitivity C-reactive protein and retinol binding protein 4 be associated with the deterioration and progress of coronary atherosclerotic.2.There isn't statistical significance difference in serum concentration of high-sensitivity C-reactive protein and retinol binding protein 4 between between the single coronary artery stenosis and the double coronary artery stenosis and the three coronary artery stenosis;Basing on these results, we suppose that there be not direct association between the serum concentration of high-sensitivity C-reactive protein and retinol binding protein 4 and the stenosis extent of coronary artery.
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