| Objective:Studying on MKR transgenic Mice with kidney damage of type 2 diabetes (Be short for high-fat MKR mice)that fed with high fat diet, to observe the changes of JiangTang YiShen decoction on the structure and function of the renal pathology, the renal MDA content, SOD and GSH-Px activity. And preliminarily investigate the effects of JiangTang YiShen decoction on the levels of blood glucose, serum insulin, structure and function of the renal pathology, and the damages of oxidative stress in the kidney of MKR mice.Methods:1. The experimental model study on MKR mice of the type 2 diabetic kidney damage:Twenty 7-week-old MKR mice were randomly divided into two groups after stratifying by the FBG and body weight.i.e,10 mice fed by normal diet, and the others fed by high fat diet. At the same time,10 C57BL/6 wild mice were selected as the control group. The three groups were fed for eight weeks. At the end of 15th week, all mice were killed to investigate the renal pathological changes, and the contents of blood glucose, glucose tolerance, blood-fat, serum insulin, blood urea nitrogen (BUN), creatinine (Cr), urine MAlb andβ2-MG were determined, respectively.2. The effects of JiangTang YiShen decoction on the renal functions and oxidative stress damages of MKR mice fed by high fat diet:Fourty 7-week-old MKR mice were randomly divided into four groups after stratifying by FBG and body weight.i.e, MKR group, model group, JiangTang YiShen decoction group(JYD), and control group. MKR group were fed by normal diet for eight weeks, and others were fed by high fat diet for eight weeks. At the 15th week, the JYD group was treated with JiangTang YiShen decoction, the control group was treated with Gliquidone Benazepril, and other two groups were treated with distilled water. All the groups were administered intragastrically once a day for thirty days. All mice were killed at the end of thirty days, and then, the content of blood glucose, serum insulin, blood urea nitrogen (BUN), creatinine (Cr), serum RBP-4 and PAI, urine UmAlb andβ2-MG, renal MDA, SOD and GSH-Px activity of each group were tested. All the data were analyzed with SPSS (America, SPSS Inc., v15.0).Results:1. The renal pathological morphology of MKR mice significantly changed on 15th week. The contents of blood glucose, glucose tolerance, blood-fat, serum insulin, blood urea nitrogen (BUN), creatinine (Cr), urine MAlb andβ2-MG in the mice fed by high fat diet were significantly higher than those in C57BL/6 wild mice and the MKR mice fed by normal diet (P <0.01).2. The changes of renal pathology in the MKR mice treated with JiangTang YiShen decoction (JYD group) were significantly improved compared with the model group. The contents of fasting blood glucose, serum insulin, blood urea nitrogen (BUN), creatinine (Cr), serum RBP-4 and PAI, urine MAlb,β2-MG, renal MDA in JYD group were significantly lower than those in model group(P<0.01), while the activity of renal SOD and GSH-Px in JYD group mice were significantly higher than model group (P<0.01).Conclusion:1. The results of MKR and wild mice showed that there were renal damages in MKR mice, and the damages were worse in MKR mice fed by high fat diet.2. JiangTang YiShen decoction might reduce the level of blood glucose and serum insulin, improve the glucose tolerance, make the renal function and kidney pathological structure better and adjust protein metabolism, etc.3. The mechanism of JiangTang YiShen decoction to improve the kidney damage of type 2 diabetes may be about interfering with the activities of oxidative stress and improving the function and pathological structure of kidney. Through inhibiting the activities of oxidative stress, increasing the activity of antioxidase and the total antioxidant capacity of renal tissue, and reducing peroxidize substances and oxidative stress level, JiangTang YiShen decoction could abate oxidative damage of renal with diabetes, improve the structure and function of renal pathology, and adjust protein metabolism, all of which could relieve renal damage and reduce the occurrence and development of diabetes. |
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