Expression And Significance Of VEGF,Ki-67 And P21 In Pterygium

Objective:To investigate the expression of VEGF,Ki-67 and p21 in pterygium as well as the correlation between their expression and clinical pathological characteristics, further explore the pathogenesis, providing the theoretical evidences for the better treatment of pterygium.Methods:Immunohistochemical S-P staining method was adopted in detecting the expression of VEGF,Ki-67 and p21 in 62 cases of pterygia and 20 cases of normal conjunctival tissues. The relationship between these markers and clinical pathological characteristics was analyzed.Results:(1) The positive expression rate of VEGF,Ki-67 in pterygia was 74.2%(46/62),66.1%(41/62) respectively and higher than those in normal conjunctival tissues(P<0.05).The positive expression rate of p21 in pterygia was 40.3%(25/62),lower than that in normal conjunctival tissues(P<0.05). (2) The expression of VEGF in 62 cases of pterygia was correlated with clinical stage and onset(P<0.05, P<0.05), and was not associated with sex ,age and occupation (P>0.05); The expression of Ki-67 was correlated with clinical stage(P<0.05), and was not associated with other clinical pathological characteristics (P>0.05); The expression of p21 was correlated with clinical stage and onset(P<0.05, P<0.05), and was not associated with other clinical pathological characteristics (P>0.05).(3) Spearman correlation analysis showed that there was a positive correlation between VEGF and Ki-67 (r=0.279, P<0.05), a negative correlation between VEGF and p21(r= -0.267,P<0.05);It also showed that there was no correlation between Ki-67and p21 (r= -0.176,P>0.05).Conclusions:(1) The overexpression of VEGF,Ki-67 and the low expression of p21 in pterygia suggest that these markers be concerned with the development and progression of pterygium.(2) The expression of VEGF and Ki-67 were increased according to clinical stage or onset , the expression of p21 was decreased according to clinical stage or onset.It suggest that these genes may play important roles in the development and recurrence of pterygium.(3) There is positive correlation between VEGF and Ki-67 as well as negative correlation between VEGF and p21.It suggest that there may be synergistic action between the two factors during the development and progression of pterygium.