The Effect Of EGF On U87 Glioma Cell Line

PurposeGlioma is a type of epithelial tissue derived from neural tumors, central nervous system is the most common primary brain tumors, various types of tumors in the brain was the first incidence, epidemiological survey, even though parts of the world differences in the incidence of glioma, but overall the incidence of primary brain tumors account for about half, and there is an upward trend in recent years.Although the clinical and basic research glioma great development, but the prognosis of patients with high-grade gliomas has not been improved, partly due to the adjustment of the host immune system glioma has not made significant progress. Previous studies showed that body fluids to patients with glioma cell immune deficiency, so the immune system not only reduces the anti-tumor immune response, also anti-tumor immune treatment more difficult. On the molecular pathogenesis of glioma therapy for a new way, at home and abroad have already implemented a variety of gene therapy programs, such as suicide gene therapy, antisense therapy, gene therapy, tumor suppressor gene therapy, inhibition of vascular gene therapy.Epidermal growth factor (Epidermal Growth Factor, EGF) is a wide range of biological functions of low molecular peptides. For many epithelial cells, stromal cells and tumor cells with a significant role in promoting growth in the number of tumors can promote tumor cell proliferation and accelerated tumor growth. EGF with the EGF receptor on the cell membrane play a role. The results show that EGF and its receptor is highly expressed in gliomas, and glioma cell proliferation, differentiation, pathological grade and prognosis.Epidermal growth factor receptor (Epidermal Growth Factor Receptor, EGFG) ligand is epidermal growth factor (EGF), transforming growth factor-α(TGF-α), both combined with the activation of EGFR tyrosine kinase, resulting in intracellular receptor itself phosphorylated tyrosine residues, thus providing a mitotic cell signaling, causing cell proliferation.In this study, the main effect of different concentrations of EGF on the U87 cell proliferation and cell cycle in order to further elaborate the mechanism of glioma provide experimental evidence.Method1.U87 cell cultureDMEM containing 10% fetal bovine serum were cultured in high glucose medium, culture conditions were 5% C02,37℃incubation. Serum medium in adherent cells for up to 90% after the passage.2. MTT assay EGF on the proliferation of U87Experimental groups:control group, EGF 0.1ng/ml, EGF1.0ng/ml, EGF 10ng/ml. The value was determined at 450 nm optical density of each hole (OD) value. Each dose group was set up five parallel samples.3. FACS determing cell cycle EGF on the U87Logarithmic phase of U87 cells will be added with different concentrations of EGF, 5% CO2,37℃incubation 1,2,7 days respectively, according to kit instructions for cell cycle4. TEM determing EGF effect on U87 cell structureU87 cells in the logarithmic growth phase, added with different concentrations of EGF,5% CO2,37℃incubation 1,2,7 days respectively, digestion, centrifugation, 2.5% glutaraldehyde fixative fixed delivery China Medical University electron microscope electron microscope center photo.5. Statistical analysisApplied Medical Statistics software SPSS 11.0 to analyze the experimental data with X±S, by t test, P<0.05.Conclusion1, EGF in the (0.1ng/ml, 1.0ng/ml) concentration on the proliferation of U87 cells. 2, EGF in the (0.1ng/ml,1.Ong/ml) concentration, the cell is in active growth state, cell pseudopodia increased...