To Study The Association Between The Metabolites Of Intestional Flora And Diabetes Mellitus In Mice

Objective:To study the Association between the metabolites of intestional flora and diabetes mellitus in mice. Methods:Animals were divided into four groups:normal group, high fat fed group, diabetes model group and treatment group (diabetes with drug prevention). Diabetic models were induced by 4w high fat diet and 150mg/kg streptozotocin injected intraperitoneally. The treatment group was giving dynamic monitoring.Blood glucose was detected at end of the 1w,2w,3w,4w,5w separately,and the animal faeces were collected at the same tiime. Stool samples was extracted with acidic water,then detected by gas chromatography for short-chain fatty acids (SCFA).Correlation between the changes of blood glucose and SCFA were examined. When t test finished,faces samples were collected from the four groups, and the difference of SCFA and D-lactic acid were observed within them. D-lactic acid were detected by uv with D-lactic acid kit.Results:â‘ Compared diabetic group with normal group and high fat group, the content of acetic acid,propionic acid and n-butyric acid decreased and showed statistically significant difference (P<0.01),D-lactic acid increased and showed statistically significant difference (P<0.01).â‘¡Compared treatment group with diabetic group of fecal samples, the content of acetic acid, propionic acid and n-butyric acid all increased and showed statistically significant difference (P<0.01),D-lactic acid decreased and showed statistically significant difference (P<0.01). Although propionic acid and n-butyric acid had increased to normal level,acetic acid was lower than the normal group and D-lactic acid was higher than normal group.â‘¢The results of dynamic monitoring suggested that there was a negatively correlation between acetic acid and blood glucose(R=0.9927, P<0.01), n-butyric acid and propionic acid have no relationship with blood glucose. Conclusions:â‘ The decrease of acetic acid,propionic acid and n-butyric acid in diabetic group suggested an imbalance of intestinal micro-ecosystem,and certain anaerobic probiotics which produce acetic acid, propionic acid and butyric acid may be absence or decrease. When givng metform,the increase of the content of acetic acid,propionic acid and n-butyric acid suggested an improvement of balance of intestnal flora.â‘¡The increase of D-lactic acid in diabetic group reflected the damage of gut barrier function in mice,and the decrease of SCFA may be an important reason for this. D-lactate may be a pathological marker, which can indicate the increase of bad intestinal bacteria and the worse of disease.â‘¢The results of dynamic monitoring suggested that acetic acid and were closely linked with diabetes mellitus in mice.