The SiRNA Targeted To C-myc Affect Gallbladder Cancer Cells' Migration And Invasion Ability

objective The role of c-myc gene in metastasis of gallbladder cancer was investigated by observation of the mobility and invasion of gallbladder cancer cells in vitro, in order to provide the evidences for selecting molecular targets in interfering gallbladder cancer metastasis.Methods the expression of c-myc gene was determined by real time PCR and western blot in parental gallbladder cancer cell line GBC-SD and its highly metastatic subpopulation GBC-SD/M3. The siRNA targeted to c-myc were transfected into GBC-SD/M3, and then the migration and invasion abilities of GBC-SD/M3 cells were detected by in vitro transwell assays. The changes of the downstream signal of c-myc were determined by western blot assays.Results the expression of c-myc gene was up-regulated in highly metastatic subpopulation, compared with that in its parental gallbladder cancer cell line. After the transfection of the c-myc siRNA in highly metastatic subpopulation, the RNA and protein levels were significantly reduced. Meanwhile, the migration and invasion abilities of the GBC-SD/M3 cells were inhibited. The detection of the downstream signal pathway of c-myc indicated LIN28 was reduced after the transfection of c-myc siRNA.Conclusion c-myc was up-regulated in the GBC-SD/M3 compared with GBC-SD. c-myc siRNA reduced the migration and invasion of gallbladder cancer cells. The inhibition may involve the down-regulation of LIN28.