| Myrica rubra Sieb.et Zucc., belonging to Myricaceae family, is a specific fruit species in China. The cultivated area of Myrica rubra in China is more than 99% in the world, including Zhejiang Province, where the cultivated area, yield, variety and quality rank first in China. At present, the food industry based on Myrica rubra resources has a certain scale, while the utilization of Myrica rubra marc is still blank. Therefore, Myrica rubra marc were chemically studied in our research project for serching for bioactivities compounds.7 compounds were isolated and purified from Myrica rubra marc. Their structures were determined by UV, MS and NMR analysis, respectively. Two (compound 1 and compound 6) of them are isolated from this family for the first time. In addition, anti-oxidation activities of some of the compounds had completed. 1. The chemical composition and the pharmacological actions of components from Myricaceae were reviewed, which can provide the guideline for the research of Myricaceae.2. Four fractions were acquired from Myrica rubra marc by a series of steps including extraction and concentration, followed by extraction with petroleum ether, ethyl acetate and n-butanol. The anti-oxidative activities of the extracts were evaluated in vitro, including DPPH radical scavenging activity, Fenton action and reducing power methods. The results showed that ethyl acetate fraction and n-butanol fraction of Myrica rubra marc crude extracts exhibited strong scavenging abilities for DPPH free radical, and their half-inhibitory concentration (IC50) were 53μg/mL and 75μg/mL, respectively, which were approximately 1.83 times and 1.30 times as much as that of Vitamin C (97μg/mL).3. The chemical constituents in the ethyl acetate extract of Myrica rubra marc were separated by chromatography on silica gel, Sephadex LH-20 and Pre-HPLC to yield five compounds:cabraleone(compound 1), quercetin(compound 2), citric acid(compound 3), myricetin 3-O-α-L-rhamnopyranoside(compound 4) and quercetin 3-O-β-L-rhamnopyranoside(compound 5).4. The chemical constituent in the n-butanol extract of Myrica rubra marc was isolated by HSCCC, with the solvent system of ethyl acetate-n-butanol-water=4:1:5. And then, B1 and B2 were acquired. The fraction B1 was isolated by Sephadex LH-20 to obtain neohesperidin (compound 6). The fraction B2 was isolated by Sephadex LH-20 to obtain n-butyl-β-D-fructopyranoside(compound 7).5. Reserch on anti-oxidation of some of compouds from Myrica rubra marc:DPPH radical scavenging activity had been done to ecaluate anti-oxidation of some of the compouds isolated from Myrica rubra marc. The results showed that the scavenging capacity of compound 4 (IC50=0.38μg/mL) and compound 5 (IC50=0.58μg/mL) are 6.11 times and 4.00 times as that of VE respectively, and compoud 2 is already more than 94% when the concentration is 25.0μg/mL, which is much stronger than Vitamin E, compoud 4 and compoud 5. It is concluded that they showed good antioxidant activities.The results of present study will be useful to further phytochemical investigation on Myrica rubra.
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