The Experimental Study Of The Acupuncture Oxygen Therapy Influencing Inflammatory Cytokines Of Rabbits Chronic Rhinosinusitis

Objective:From the influence factor of inflammatory cytokines, reveal molecular biology mechanism of acupuncture oxygen therapy for chronic rhinosinusitis.Methods:1.5～3.0kg,63 healthy New Zealand white rabbits. According to the biological safety standardsⅡlevel raised for 1 week, according to the sequence of Numbers, using SAS software to divide into:the model group, the normal group, the TCM group, the western medicine group, the acupuncture group, the sham-operation group, the acupuncture oxygen group. From 43 days after built model, the TCM group served soup, the western medicine group served clarithromycin, the acupuncture group needled, the acupuncture oxygen based on needled with oxygen.2 weeks after treatment, get blood from heart, killed animals, remove the nasal mucosa. With ELISA test rabbits serum IL-8, TNF-a, using Real-time PCR test IL-8mRNA. To observe the nasal mucosa under the microscope.Results:1.The model group rabbits serum IL-8, TNF-a compared with the normal group significant difference. The acupuncture oxygen group serum IL-8 compared to the model group not difference. The acupuncture oxygen group serum TNF-a compared with the model group difference.2. The model group nasal mucosa IL-8mRNA compared with the normal group difference.The acupuncture oxygen group nasal mucosa IL-8mRNA compared to the model group difference.3. Under the microscope, the model group rabbits visible nasal mucosa epithelial cells, cilia and epithelial cells arranged disorder, and small ulcers. The acupuncture oxygen group of nasal mucosal inflammation repairs damage.Conelus i on:The acupuncture oxygen group effectively improve rabbits body caused. The acupuncture oxygen group reduced serum IL-8, TNF-a concentration. The acupuncture oxygen group reduced the rabbit nasal mucosa IL-8mRNA expression. The acupuncture oxygen group of nasal mucosal inflammation repairs damage.