Effects Of Chlorotributylstannane Tributyltin Chloride On The Mucosal Immunity Of Carp (Cyprinus Carpio L.)

Chlorotributylstannane Tributyltin chloride is a kind of organictin compounds, and which is toxic and refractory. It widely spreads in the water environment and can bring serious harm to the respiratory system, nervous systerm, reproductive system and immune system.In this study, the median lethal concentration of Chlorotributylstannane Tributyltin chloride in 48 hours was done research with carp (Cyprinus carpio L.) to evalue the toxicity of Chlorotributylstannane Tributyltin chloride, and themedian lethal concentration is 7.62μg/L. The result suggests that Chlorotributylstannane Tributyltin chloride has strong toxicity to carp.Treated the carp with Chlorotributylstannane Tributyltin chloride of different concentrations (0μg/L, 0.1μg/L, 1.0μg/L and 3.0μg /L) for 28 days and then collected blood. Diluted the blood and counted the number of WBC. Compared with the control, the number of WBC had no significant difference in the low concentration group, but the number significantly reduced in the middle concentration group and decreased most in the high concentration.Treated the carp with Chlorotributylstannane Tributyltin chloride of different concentrations for 28 days and then collected blood and prepared for serum, following with detecting the complement activity in the serum. In this experiment, sheep red blood cells were used as antigen, and reacted with anti-sheep erythrocyte antibody with the preparation of serum, which was used as complement source, and then counted the complement activity according with the percentage hemolysis. The statistical ananlysis showed that the lowest group had no significant different compared with the control, the complement activity of the midlle group decreasted significantly, and the chang of the high concentration group was most significant.Sampled randomly 3 fish from each of group, and intraperitonael injected of sheep red blood cells. Eleven days post-SRBC immunization carp were sacrificed, head kidney cells suspended in Hank's solution at 2×10~7 cells/ml, and a modified Jerne plaque assay performed to determind antibody forming cell (AFC) numbers. Each hemolytic plaque represented an AFC. The statistical ananlysis showed that the AFC numbers had not been affect in the low and middle concerntration groups, while the numbers decreased significantly in the high concertration group.Immuned each group carp with inactivated Aeromonas hydrophila, at the time of before and after stimulation 6 24, 72, 120 and 168 hours, took out of 3, and sacrificed. Extract RNA of each tissue and reversed to cDNA. Detected the expression of hepcidin, MHCⅡβand pIgR mRNA in spleen, forgut and hindgut with PCR. The results suggested that the three genes'expression had not been effected in the low concentration group, the expression was only been effected on the peak value in the middle concentration group, and the expression was been effected at all the time in the high concentration group.