Relationship Of Toll-Like Receptor-4,Human β-Defensin-2 In HBV Intr-Auterine Infection Placenta And IFN-γ In The Maternal Blood

Object: to research Toll-like receptor-4(TLR-4), Humanβ-defensin-2 (HBD-2) andγ-interferon (IFN-γ) in the role of HBV intrauterine infection.Method: Selected 57 pragnant women with HBsAg, HBeAg positive, normal liver function of peripheral blood and HBV-DNA levels measured before delivery. In the case of informed consent, taking 3 ml of cord blood sent to laboratory to detect the HBV markers when diliverying, According to the results, either HBsAg or HBeAg is positive for the intrauterine infection group and negative for uninfected group. Choose 20 cases of pregnant women with HBVM normal for the control group in the same period.taking 3 ml peripheral blood before delivery for determination of IFN-γfor all cases. after delivery, sterilely collecting processed placenta and after 15 minutes putting it in liquid nitrogen tank, storing in -80℃freezer. We tested HBV-DNA of pregnant women in placental tissue by PCR. We tested the mRNA of TLR-4, HBD-2 in placenta using real-time reverse transcription polymerase chain reactions by dsDNA- binding dyes of SYBR Green, which calculated the relative expression using 2-△△Ct method. Results: (1) The HBV intrauterine infection rate of HBsAg, HBeAg positive in maternal serum was 45.6%. (2) comparing between HBVDNA≥1×10~5 and <1×10~5, Differences of the rate in intrauterine infection have significant,χ~2=29.317.(3)The placental HBV infection rate was 63.16%. (4) In the intrauterine infection group, IFN-γlevels in maternal blood were lower than the uninfected group and the control group (p<0.01), Differences between the uninfected group and the control group were not significant (p>0.05). In the intrauterine infection group, the expression of TLR-4 mRNA was lower than the uninfected group and the control group (p<0.01). Differences between the uninfected group and the control group have no significant (p>0.05). In the intrauterine infection group, the expression of HBD2 mRNA was lower than the uninfected group and the control group (p<0.01), Differences between the uninfected group and the control group were not significant (p>0.05). (5) The expressions of TLR-4 mRNA in placental and IFN-γlevels in peripheral blood of pregnant women have a significant positive correlation (r=0.815, p<0.01). Expressions of HBD2 mRNA in placental and IFN-γlevels in peripheral blood of pregnant women also have a significant positive correlation (r=0.685, p<0.01). Expressions of TLR-4 mRNA and HBD-2 mRNA have a significant positive correlation (r=0.896, p <0.01).Conclusions: (1) HBsAg, HBeAg positive and HBV-DNA≥1×10~5 copies/ ml in maternal serum is easy to transmit the virus to the fetus. (2) One way of hepatitis B virus intrauterine infection is by placenta. (3) The expression of TLR-4, HBD-2 in placenta and IFN-γin pregnant of peripheral blood are downward in the newborn with Hepatitis B virus intrauterine infection, Causing the body to resist the virus weakened. (4) Maternal IFN-γlevels have positive correlations with the expression of TLR-4, HBD-2. The expressions between TLR-4 and HBD-2 in placenta have a positive correlation. Their expressional descending possible commonly lead to intrauterine infection of hepatitis B virus, in the whole cascade signaling pathways.