| Objective: The establishment of diet-induced obesity model was given high-fat and high-calorie diet. To investigate the effect of dietary-induced obesity on the testicular spermatogenic cell apoptosis in pubertal male rats.Method: Forty health male rats were divided randomly into a control group and a high-fat group. The rats in control group (n=20) were fed on normal diet; the high-fat group (n=20) was given high-fat and high-calorie diet. The rats had been killed in ten weeks, and their testis had been obtained. Peripheral blood total cholesterol (TC), Triglyceride (TG), High-density lipoprotein cholesterol (HDL-C) and Low-density lipoprotein cholesterol (LDL-C) were detected by automatic biochemical analyzer (ACA). The pathological changes of testis were observed by light microscope. The apoptosis of testis cell was detected by TUNEL staining. Immunohistochemistry was adopted to detect the expression of Bcl-2 and Bax protein in testis. The expression of Bcl-2 mRNA and Bax mRNA in testis was detected by RT-PCR.Result: Result: 1. The establishment of diet-induced obesity model: The rats in control group were fed on normal diet; the high-fat group was given high-fat and high-calorie diet. .The body weight in experimental group was higher 20% than that in control group at ten weeks old, Successfully established diet-induced obesity model. TC, TG, LDL-C and HDL-C (5.17±0.17, 1.18±0.09, 1.76±0.11, 5.08±0.18) in the high-fat group were obviously higher than the control group (1.38±0.12, 0.39±0.05, 0.97±0.07, 0.75±0.06), and the discrepancy was significant (P<0.05).2. Pathological observation of rat testis: In the ten weeks old, the control group rats'seminiferous tubule developed well, spermatogenic cell has arranged compact, frame distinct, the proliferation of spermatogenous cells which paste to end epidermis basal membrane were good, there are include spermatogenous cell, primary spermatoeyte and spermoblast of every developmental stage cells in the tubule. In the end of experiment, the tubule was developing full maturity. The tubule wall was thinning, cells arranged in disorder and displacement which away from the basement membrane of seminiferous tubules after four weeks, the sperm was decreased in the ten weeks old. In the end, the arrangement of spermatogenic epithelium cells were not in order, spermatogenic cellular layers were reduced and the number of mature sperm decreased obviously.3. Apoptotic cells in rat testis situation: The apoptotic index of spermatogenic cells in high-fat group (37.17±2.74) was definitely higher than the control group (5.16±0.81, P<0.01). The spermatogenic cells in apoptosis were mainly spermatogonia and spermatocytes.4. Rat testis in Bcl-2, Bax protein expression: The expression of Bax protein (153.26±8.74) in high-fat group was definitely higher than the control group (101.81±6.14, P<0.01). The expression of Bcl-2 protein (139.26±7.21) in high-fat group was definitely lower than the control group (159.37±8.96, P<0.01).5. Rat testis Bcl-2mRNA, BaxmRNA expression: The expression of Bax mRNA (1.08±0.12) in high-fat group was definitely higher than the control group (0.37±0.04, P<0.01). The expression of Bcl-2 mRNA (0.46±0.05) in high-fat group was definitely lower than the control group (1.05±0.11, P<0.01).Conclusion: 1. The establishment of diet-induced obesity model was given high-fat and high-calorie diet, alimentary obesity in puberal can lead to the microstructure of testis disorder.2. The spermatogenic cells in apoptosis were mainly spermatogonia and spermatocytes. the primary spermatoeytes were transformed disorder, and then the numer of spermatoblasts and sperms were reduced.3. Alimentary obesity can induce apoptosis of spermatogenic cells in testis increasing. The mechanism may associated with decreasing in expression of Bcl-2 and increasing in expression of Bax.
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