Effect Of Indoleamine 2,3-dioxygenase In Biological Behaviour Of HepG2 Hepatocellular Carcinoma Cells

Objective: To study the effect of indoleamine 2, 3 -dioxygenase on biological behaviour of hepatocellular carcinoma cells.Method: pcDNA3.1-IDO-HepG2 cells for the text group,the HepG2 cell and HepG2 cell transfected with blank plasmid pcDNA3.1 (pcDNA3.1HepG2)was used as control group. Three cells culture process of the morphological changes observed. Changes of HepG2 transfected by pcDNA-3.1/ pcDNA-3.1-IDO were detected by MTT, FCM, cell adhesion assay and tramswell chamber assay in vitro. HepG2, pcDNA3.1HepG2 and pcDNA3.1IDO-HepG2 cells were studied respectively.Results: Compared with the HepG2 and pcDNA3.1HepG2 cells, pcDNA3.1-IDO-HepG2 cells became longer and had more filopodia. FCM showed that the growth of HepG2, pcDNA3.1HepG cells were blocked in G0 /G1 stage; Attachment assay showed that HepG2, pcDNA3.1HepG cells (0.93±0.18,1.07±0.14 ) adhered more slowly to Matrigel than pcDNA3.1IDO-HepG2 cells(1.179±0.36) (P<0.01). By transwell chamber system, the capability of invasion of pcDNA3.1IDO-HepG2 (269.00±21.60)cells was more significantly increased than HepG2, pcDNA3.1HepG cells(185. 60±15.58, 198.80±12.60). The quantity of the pcDNA3.1 IDO-HepG2 cells breaking through the matrigel increased significantly ( P < 0. 01).Conclusion: The tranfection of IDO gene could increase the HepG2 cell′s capability of proliferation, adhesion and invasion.