Effects Of Bushenyangjing Decoction On AQP9 Expression In Ovary, Uterus Of Rats With PCO

Abstract:Objective: Using the polycystic ovary (PCO) rats model induced by Sodium Prasterone Sulfate as the study object,to investigate the relationship between aquaporin9(AQP9) and pathology change of ovary and uterus in PCO rats and observe the influence of bushenyangjing decoction on AQP9 expression in ovary and uterus of rats with PCO and investigate the mechanism of bushenyangjing decoction treating PCOS.Methods:64 SD female rats,23 days age,were divided randomly into normal control group including 16 rats and PCO model group including 48 rats which were induced by sodium prasterone sulfate.Then the model rats were divided randomly into model control group,clomifene citrate group,high dosage of bushenyangjing decoction group,middle dosage of bushenyangjing decoction group and low dosage of bushenyangjing decoction group.After treating and killing the animals,the expression of AQP9 in the granulosa cells and endometrial epithelial cells with immunohistochemistry streptavidin peroxidase conjugated method was examined and observed by optical density value with microscope and analyzed by computer imaging technique software named Image-Pro plus 6.0 to compare the differences among these groups. Results:1.Through imaging and statistics analyzing,AQP9 expresses in granulosa cells of follicles and corpus luteum of polycystic ovary in all the groups including model, normal control group, ,high,middle and low dosage of bushenyangjing decoction and clomifene citrate group. IHC showed AQP9 was detected in the cytomembrane and cytoplasm of granulosa cells.The OD expression of AQP9 in model group was much smaller than that in normal control group(P<0.01);The OD expressions of AQP9 in all the medicine groups including the high, middle and low dosage of bushenyangjing decoction and clomifene citrate group were much greater than that in model group (P<0.05); The OD expression of AQP9 in the high dosage of bushenyangjing decoction group was much greater than that in the clomifene citrate group (P<0.01), that in the middle and low dosage of bushenyangjing decoction were little greater than that in the clomifene citrate group(P>0.05);The OD expressions of AQP9 in the high dosage of bushenyangjing decoction group was much greater than that in the low dosage group(P<0.01), but that in the middle dosage of bushenyangjing decoction were little greater than that in the low (P>0.05); The OD expression of AQP9 in the high dosage of bushenyangjing decoction group was much greater than that in the middle dosage group(P<0.01).2.AQP9 expresses in the cells of uterine epithelium in all the groups including model, normal control group,clomifene citrate group,high,middle and low dosage of bushenyangjing decoction. IHC showed AQP9 was detected in the glandular epithelium and additionally in the luminal epithelium of the rat uterus. The OD expression of AQP9 in model group was much smaller than that in normal control group(P<0.01);The OD expressions of AQP9 in all the medicine groups including the high, middle and low dosage of bushenyangjing decoction and clomifene citrate group were much greater than that in model group (P<0.05); The OD expression of AQP9 in the high dosage of bushenyangjing decoction group was much greater than that in the clomifene citrate group (P<0.01), that in the middle and low dosage of bushenyangjing decoction were little greater than that in the clomifene citrate group(P>0.05);The OD expression of AQP9 in the high dosage of bushenyangjing decoction group was much greater than that in the low dosage group(P<0.01), but that in the middle dosage of bushenyang- jing decoction was little greater than that in the low one(P>0.05); The OD expression of AQP9 in the high dosage of bushenyangjing decoction group was much greater than that in the middle dosage group(P<0.01).Conclusion: 1.The expression of AQP9 in ovarine granulosa cells of follicles and corpus luteum decreased obviously in PCO model group,then we infer that AQP9 may be related to the development of follicles. 2.The expression of AQP9 in glandular epithelium and luminal epithelium of the rat uterus decreased obviously in PCO model group,then we infer that AQP9 may be related to the endometrial receptivity.3. Bushenyangjing decoction can increase the expression level of AQP9 in granulosa cells and uterine glandular epithelium and luminal epithelium in PCO model group for the purpose of treating PCOS through regulating the follicular development and endometrial receptivity.