The Differential Somatostastion Receptors Of Octreotide On The Expression Of Human Lung Cancer A549 Cells

Objective To investigate the mRNA and protein expression of somatostatin receptors in human lung cancer A549 cells lines and to explore the mRNA and protein expression of somatostatin receptors with octreotide acting on the human lung cancer A549 cellsand to analyse the clinical significance of the changed expression and the influence of ocetreotide on the proliferation of human lung cancer A549 cells.Methods RT-PCR and immunocytochemistry were used to detect mRNA and protein expression of somatostatin receptors in human lung cancer A549 cells and to carry out semiquantitative analysis. MTT were used to evaluate the antiproliferative effect of octreotide.Results The effect of SSTR1-5 on the human lung cancer A549 cells was verified. Octreotide could inhibit the growth of A549 cells in dose dependent manner. The mRNA expression of all 5 subtypes of somatostatin receptors was different. SSTR3 and SSTR1 mRNA expressions were significantly higher than SSTR2,SSTR4,SSTR5 (p<0.05). The mRNA and protein expression of SSTR1 were significantly higher than SSTR2,3,4,5 with octreotide acting on the cells (P<0.05). The mRNA and protein expression of SSTR2 and SSTR5 were significantly higher than control group (P<0.05).Conclusion 1.All 5 subtypes of somatostatin receptors expression in human lung cancer A549 cells. 2.The proliferation of A549 celle is reduced by octreotide. 3.SSTR1,SSTR2 and SSTR5 play an important role in inhibiting human lung cancer A549 cells growth and proliferation.