The Effects Of Yishen Decoction On Endothelin-1 (ET-1) And Angiotension Ⅱ (AngⅡ) Of Renal Interstitial Fibrosis Model Induced By Adenine In Rats

Objective:To investigate the effect of the Yishen Decoction on ET-1, Angâ…¡and kidney tissue of renal interstitial fibrosis rats caused by adenine, and illustrate the mechanism of Yishen Decoction on delaying renal interstitial fibrosis through the experiment.Methods:Thirty-Two male SD rats were randomly divided into 4groups: normal group, model group, YiShen Decoction group and Enalapril group. In addition to the normal group, the renal interstitial fibrosis model of the rest groups were established by intragastric administration of adenine after one week adjusting feeding. In the experiment, the common physical situation and weight of the rats were observed. On the 7th,4th and 21th day, SCr and BUN levels were checked through cutting the tail and taking blood. On the 21rst day, the rats were killed and serum was adopted to check SCr,BUN,RBC,Hb,HE and Masson staining methods were adopted to observe the pathologic change of the experimental rats'kidney tissue and the ET-1 and Angâ…¡in nephridial tissue was expressed by the Immunohistochemistry observation. Results:1. The physical status and symptom of the RIF rats were obvious ly improved and the weight were increased in YiShen Decoction group there was significant difference(p<0.01) at each time point between YiShe n Decoction group and model group, On the 21rst day, the effect in Yish en Decoction group is better than that in enalapril group (P<0.05).2. Yish en Decoction group has obvious different effect on increasing RBC, Hb a nd reducing Scr, BUN compared with the model group(P<0.01). On the 2 lrst day, the effect in Yishen Decoction group is better than that in enala pril group (P<0.05).3. Cell morphology observation:the normal group s hows no abnormality, while the renal interstitial injury and inflammatory cell infiltration of the model group are serious compared with two treatm entgroups, and the difference between them was statistically significant (p <0.01). There is no statistically significant difference between the two tre atment groups (p>0.05).4. Compared with the model group, ET-1 and A ngâ…¡levels of the two treatment groups decreased, the difference between groups was statistically significant.Conclusion:YiShen Decoction could decrease ET-1 and Angâ…¡levels and improve the renal interstitial fibrosis to reduce and delay theprocess of c hronic renal failure.