The Construction Of Several Decalcified Bone Matrix Scaffold And Study To Its Acivity Of Osteogenesis

Objective:To fabricate various decalcified bone matrix (DBM) through improved methods with alligeneic bone of rabbits (constructing with physical process to the DBM or compounding active cytokine to the DBM) in order to observe and investigate the reparation of them to the bone defect. The other objective is to detect whether our various DBM can do as scaffold of bone tissue engineering and carrier of active cytokine. The last objective is to provide a new thought of fabrication of-scaffold to bone bank. Methods:First, alligeneic bone are deal with through dehydration, degrease and refrigeration under profound hypothermia to execute portion DBM (PDBM), profound hypothermia refrigeration PDBM (PHRPDBM) and profound hypothermia refrigeration bone (PHRB) and they are all 1.5 cm long. All the material is stored hermetically and sterilized under 60CO. Then, the material is disparted into 8 groups and they are tubular DBM (TDBM), splitting no tubular DBM (SNTDBM), TDBM compounding b-FGF (TDBM+b-FGF), splitting no tubular DBM compounding b-FGF (SNTDBM+b-FGF), tubular DBM compounding autgenous bone marrow (TDBM+ABM), the blank reference (TBR), tubular profound hypothermia refrigeration bone (TPHRB) and tubular profound hypothermia refrigeration DBM (TPHRDBM). The materials are planted into the defects of middle superior segment of radius each and the length of defects are 1.5 cm either. The last step is to kill 2 rabbits partly at 1,2,4,8,12 weeks after implantation each groups and draw materials to detect indes of observation of specimen, X-rays, slice of histology, alkaline phosphatase (ALP) of callus and content of metal element of callus. The image of X-rays and slice of histology are analyzed and changed-over with variable and the result with the data of ALP and metal element is managed with analysis of variance of single factor or double factor. Results:(1) The TDBM has the more activity of osteogenesis than SNTDBM (P<0.05); (2) TDBM+b-FGF has the more activity of osteogenesis than SNTDBM+b-FGF(P<0.05); (3) TDBM+ABM has the more activity of osteogenesis than the ones which no ABM (TDBM) (P<0.05); (4) The bone defects of TBR of every time point don't heal all and form fibrous union. The bone defects of other groups all heal with bone; (5) PHRDBM has the more activity of osteogenesis than the single PHRB(P<0.05); (6) TDBM+b-FGF has the more activity of osteogenesis than the ones no b-FGF(P<0.05); (7) PHRDBM has the more activity of osteogenesis than the single DBM(P<0.05); (8) PHRB has low antigenicity, hasn't or has low inductive ability of osteogenesis only but it has good ability of osteogenesis of guidance and conduction. Conclusion:(1) The improved methods of fabrication of DBM, PHRB and PHRDBM in this topic are simple and can be make easily. The scaffolds of construction can be applied as operative material of restoring defect of bone. (2) Dosage of sterilization in this topic is safe and effective and this dosage reserve the material's inductive activity of osteogenesis. (3) The reason of TDBM having more activity of osteogenesis than SNTDBM may root from mechanism of guiding bone regeneration (GBR). (4) TDBM has the primary function of carrier of active cytokine. (5) The reason of PHRDBM having more activity of osteogenesis than single DBM may root from its lower immunogenicity. (6) PHRB is fit for operative scaffold of restoring defect of bone in section of weight loading even more.