The Relationships Between Expression Of Cyclooxygenase-2 And Vascular Endothelial Growth Factor And Radiosensitivity Of Esophageal Squamous Cell Carcinoma Carcinoma

ObjectiveThrough observing the expression of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) in esophageal carcinoma tisssue and normal mucous membrane of esophagus tissue respectively, and analysis the relationship between the COX-2 and VEGF expression levels in esophageal carcinoma tisssue with the clinicopathologic characteristic of esophageal carcinoma, and the relationship between the expression of each of them and simple radiotherapy effect in patients with esophageal carcinoma, in order to explore some predictors of radiosensitivity in patients and offer the theoretical foundation for formulating reasonable individualized treatment perscription.Methods60 cases of esophagus squamous cell carcinoma tissue sample.which were confirmed by esophageal speculum biopsy before radiotherapy and 20 cases of normal mucous membrane of esophagus tissue sample were collected. Express-ions of COX-2 and VEGF were observed by streptavidin-peroxidase (SP) immun-ohistochemical staining method.60 patients with esophageal carcinoma were received conventional fractionated radiation therapy by 6MV-X ray linear accelerator, and then evaluate the therapeutic effect when the radiotherapy com-pleted and 3 months after radiotherapy through reference the X-ray film evaluation criterion. The results were treated by statistics methods of Chi-square test, Spearman Correlation analysis and logistic regression analysis.Results1,The expression of COX-2 and VEGF in in esophageal carcinoma tisssueOf 60 cases esophageal carcinoma tisssue, the positive rates of COX-2 were 68.3%, the positive rates of VEGF were 76.7%; and of 20 cases normal mucous membrane of esophagus tissue, the positive rates of COX-2 and VEGF were 0 and 11.1% respectively. There were significant differences(P<0.05) at esophageal carcinoma tisssue and normal mucous membrane of esophagus tissue. Of 60 cases esophageal carcinoma,35 cases were positive expression of both COX-2 and VEGF, while 8 cases were negative expression of both of them. Through statistics analysis, there was close positive correlation between COX-2 and VEGF expression.2,The relationship between COX-2 and VEGF expression levels with the clinicopathologic characteristic of esophageal carcinomaThe expression of COX-2 had no relationship with the patients's sex, age, clinical stage, histological differentiation, the depth of tumor and lymph node metastasis, there were no statistically significant (P<0.05)The expression of VEGF had a positive correlation with the district lymph node metastasis state, and the positive rates of VEGF in the group with lymph node metastasis were higher than the group without lymph node metastasis. The VEGF expression levels had no relationship with the patients's sex, age, clinical stage, histological differentiation, the depth of tumor, there were no statistically significant (P<0.05)3,The relationship between COX-2 and VEGF expression levels with the radiotherapy effect of esophageal carcinomaOf 60 cases of esophageal carcinoma, the radiotherapy availabiliy rate of tumors with COX-2 negative expression were 94.7%, and the availabiliy rate of COX-2 positive expression were 61.0%, andχ2=7.288, P=0.007, there was significant difference. The radiotherapy availabiliy rate of tumors with VEGF negative and positive expression were respectively 100.0% and 63.0%, andχ2=5.514, P=0.019, the difference was signiticant.4,The multiplicity analysis of influence factor to radiotherapy effect of esophageal carcinomaBy noncondition logistic multiple factor regression analysis, the signifant influence response of tumor to radiotherapy were clinical stage, the expression of COX-2 and VEGF, the OR value were 16.518,6.978,4.423.Conclusions1,The expression of COX-2 and VEGF were both high-grade in esophageal squamous cell carcinoma, non-expression or low-expression in normal mucous membrane of esophagus, and show that both the COX-2 and VEGF may have relationship with the carcinogenesis and development of esophageal carcinoma.2,The COX-2 and VEGF expression in esophageal carcinoma were significant positive correlation. It is indicated that there is a certain regulation between COX-2 and VEGF, the COX-2 may induce the expression of VEGF, and then effect the tumor new vascularization and tumor growth.3,The expression of COX-2 had no relationship with the clinicopathologic characteristic of esophageal carcinoma. The VEGF had a positive correlation with the district lymph node metastasis state, and the positive rates of VEGF in the group with lymph node metastasis were higher. It showed that VEGF over-expression provids referencing index for metastasis of esophageal carcino-ma.4,The positive expression of COX-2 and VEGF in esophageal carcinoma were both had negative correlation with the radiotherapy effect, patients with negative expressions of COX-2 and VEGF were more radiosensitive than those. with positive expressions. It is said that both COX-2 and VEGF were important referencing index that could reflect the radiosensitivity and radiotherapy effect in the near future for esophageal carcinoma. Through detecting the expression of them, doctors can formulate a individually therapy plan for different patients, and also it provide a theoretical foundation that use radiotherapy combining selective COX-2 inhibitor and antiangiogenic therapy for the patients with COX-2 and VEGF over-expression.5,By noncondition logistic multiple factor regression analysis, the signifant influence response of tumor to radiotherapy were clinical stage, the expression of COX-2 and VEGF, the OR value were 16.518,6.978,4.423.