Studies On The Apoptosis Of Trauma After Spinal Cord Injury In Bufo Gargarizans

Spinal cord injury often results loss of voluntary motor and sensory function below the site of injury. The long-term neuro deficits after spinal cord injury may be due in part to widespread apoptosis of neurons. Apoptosis is a morphologically defined form of programmed cell death seen in a variety of circumstances. Many factors involved in the process of Apoptosis include Caspase-3, Bax, Bcl-2 and c-kit. The Caspase-3 is one of cysteine proteases regulates the execution of the mammalian apoptotic cell death program; Bax and Bcl-2 belongs to Bcl-2 family, Bax promotes apoptotic, but Bcl-2 promote cell survival by inhibiting adapters needed for activation of the proteases (Caspases) that dismantle the cell; c-kit is one of proto-oncogenes which inhibits the expression of apoptotic gene. In addition, some genes that take part in apoptosis are unkown by know, maybe they play an important part in apoptosis after spinal cord injury.Immunohistochemistry technology was used to investigate the expression of genes related to apoptosis and c-kit in spinal cord injury (SCI) in toad, The results were shown as follows: (1) With the increase of time after SCI, the numbers of Caspase-3 and Bax which apoptotic cytokines were increased at first and then decreased; (2) The expression of Bcl-2 increased first and then decreased; (3) The ratio of Bcl-2/Bax put out decrease-increase trend opposite to that of the Caspase-3 and Bax, maybe the raising of the ratio restrained the expression of Caspase-3; (4) The expression of c-kit reached maximum till the expression of Bcl-2 increased and Bax decreased, It is suggested that c-kit restrain apoptosis and protect the neurons. Perhaps these differences help the toad recover from damage, and was one of the reasons that lower vertebrates had stronger recycled ability than the higher ones.The RNA of toad was extracted using Trizol method, RAPD primers were screened by optimized RAPD procedure. The most suitable primer group was screened from 20 random primers and 3 anchor primers. These polymorphic primers could be used in the study of spinal cord injury by mRNA dfferential display in toad.