Enhancement Of Taxane Production Induced By UV-B Radiation In The Needles Of Taxus Chinensis Var. Mairei And The Design Of The Device For UV Radiation

Paclitaxel is considered to be one of the most effective anticancer drugs available today. For its remarkable antineoplatstic effect and unique mechanism, paclitaxel is used in the treatment of many kinds of cancers. The low yield of paclitaxel is the key factor of the widely use for paclitaxel treatment. To overcome this problem many methods are employed to enhance the production of paclitaxel, however, there is still some elusive points.UV-B radiation could activate the enzyme associated with secondary metabolism in medicinal plants, and the content of active compounds is also improved. In this paper, to enhance the content of paclitaxel and 10-deacetybaccatin III in the needles of Taxus chinensis var.mairei, the freshly harvested needles of Taxus of Chinese marin var. are treated with UV-B irradiation. At first, we access the changes of content of paclitaxel and 10-deacetybaccatin III in needles of taxus under the condition of different UV-B intensity and irradiation time. In addition, we also evaluated the time-manner of incubation time on the paclitaxel and 10-deacetybaccatin III production followed UV-B irradiation with the optional UV-B intensity. The results are analyzed with the two-way analysis of variance. We found that when the UV-B intensity was 91.46μW cm-2, the increase of paclitaxel and 10-deacetybaccatin III content reach the maximum, and increased by 79.7% and 83.3% respectively. With the optional UV-B intensity, the content of paclitaxel reaches the maximum increase 14.5% when irradiated for 6h and incubated for 72h. However the content of 10-deacetybaccatin III reaches its maximum increase 21.4% when irradiated for 2h and incubated for 48h.During the experiments, a simple device is used which couldn't regulated accurately the factors affected the experiment results. To overcome this problem, we designed a device based on the LPC2478 microprocessor which could accurately measure and regulate the UV radiation intensity, ozone content, temperature and humidity in the environment. In this paper, the design of the hardware and software was mainly introduced. The Hardware includes the UV intensity measurement and regulates modules, ozone measurement and control module, temperature measurement and control module and humidity measurement and control modules. Software section consists of three parts, including key scan module, detection of environmental factors sensors module and the drive of the control components of environmental factors module.