Preparation And Study Of Calcium Alginate/Chitosan Microsphere Loaded With Cyclosporine

As drug carrier, microspheres in the nature of targeted and controlled-release which can improve therapeutic index, reduce drug toxicity and extend drug effect. Therefore, in order to solve the problem of oral cyclosporine delivery which acted as immunosuppressant, taking the impulsive electrostatic technique as preparation technology and using alginate and chitosan of natural polysaccharideas as carrier to study the preparation and drug-release behaviour in vitro of the microsphere loaded with cyclosporine.In this paper, cyclosporine microsphere was prepared by the mild and efficient impulsive electrostatic technique. The morphology of microsphere was observed by SEM and a reversed-phase high performance liquid chromatographic (RP-HPLC) method was established for determination of the contention of cyclosporine in microsphere so as to detect entrapment efficiency. The established method is rapid with good accuracy and reproducibility which meets the requirements of analysis. The preparation was optimized by central composite design-response surface methodology and artificial neural network model, the predictions are verified by validation test. The drug-release in vitro manifest sustained-release and pH sensitive ralease characteristics. A variety of kinetic models were used to elaborate drug-release patterns. Finally drug release mechanism was confirmed as a combination result of diffusion and erosion, but diffusion played a leading role. The release behaviour was accorded with the biphasic kinetics equation.