| Objective: To investigate expression of cytokines interleukin (IL)-18,Th1 subset produced and interleukin (IL)-10,Th2 subset produced in peripheral blood of patient with hepatitis B virus infection in different stages of disease and to explore their clinical significience.Method: Peripheral blood samples were obtained from the researched groups, the patients with HBV infection which included chronic hepatitis B, Liver cirrhosis, hepatocellular carcinoma(HCC) and health controls. The researched groups were selected from inpatients in First Affiliated Hospital of Guangxi Medical University and were accordance with the diagnosis of revised jointly by Infection Branch and Liver Disease Branch of Chinese Medical Association in 2005. Patients infected with HIV , hepatitis A virus ,hepatitis C virus, hepatitis D virus, clonorchiasis and impaired the organs including heart, brain, lung. Hepatocellular carcinoma patients were not received surgery, radiotherapy, chemotherapy and immune therapy and proved by surgery and pathology or observed by CT scans. The control group was the health adult individuals with HBsAb positive. Liver function was detected by automatic biochemical analyzer. serum HBV DNA level was detected with fluorescent- quantitative PCR. HBV markers, IL-10, IL-18 were tested by ELISA.Results: (1) Compared to the controls, the level of IL-10 increased in chronic hepatitis group and HCC group (p<0.05). The increased level of IL-10 in liver cirrhosis group showed no different significantly (p>0.05). The express level of IL-10 in chronic hepatitis B was higher than that of liver fibrosis and HCC(p<0.05), but the express level of IL-10 was not statistically significant between liver fibrosis and HCC(p>0.05).(2) Compared to the controls, the level of IL-18 increased in patients with HBV infected (p<0.05). The express level of IL-18 in chronic hepatitis B group was higher than that of liver fibrosis group and HCC group(p<0.05), but the express level of IL-18 was not statistically significant between liver fibrosis and HCC(p>0.05).(3) IL-10 levels increased with elevated levels of HBV DNA replication, but there was no statistically significant between the groups(p>0.05). The expression level of IL-18 in the group A with low virus replication is lower than group B,C(p<0.05). The Spearman rank correlation Correlation Analysis showed that IL-10, IL-18 had a positive correlation to the level of HBV DNA replication(rs=0.265, p<0.05;rs=0.297, p<0.05). (4)The increased levels of IL-10 showed no statistically significant between the groups when the level of ALT increased no more than 400 U/L(p>0.05). The increased levels of IL-18 showed statistically significant between ALT<3ULN and ALT≥3 ULN(p<0.01). The Spearman rank Correlation Analysis showed that IL-10 had a positive correlation to the level of ALT(r=0.291, p<0.01). The Pearman Correlation Analysis showed that IL-18 had a positive correlation to the level of ALT(r=0.333, p<0.01). (5) There are no correlationship among TbiL, IL-10, IL-18(p>0.05).Conclusions: (1) IL-10 plays a role in immune responses that may be involved in Chronic HBV infected state and IL-18 may take part in immune responses lead to viral clearance. (2) Interleukin-10 (IL-10) might be a cytokine that has a modulatory effect on hepatic fibrogenesis. The levels of interleukin-18 in serum might be an additive prognostic indicator in liver cirrhosis.(3) The declined levels of IL-18 may increase the risk of liver cancer.(4) The levels of IL-18 have relation with The levels of IL-10.
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