Association Between Genetic Polymorphism Of IL-2 And Susceptibility To Hepatitis B、Cirrhosis And Hepatocellular Carcinoma

Objectives To investigate the distribution of polymorphisms between+114 (rs2069763 G/T) and IL-2-384(rs2069762 T/G) in interleukin-2(IL-2) gene. To reveal the association between SNPs of IL-2 and hepatitis B、cirrhosis and hepatocellular carcinoma in GuangXi population. Explore the relationship of IL-2 single nucleotide polymorphism (SNP) and hepatitis B, liver cirrhosis, liver cancer.Methods Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) strategy and DNA sequencing methods were used to analyze polymorphisms of IL-16 in 115 patients with CHB,67 patients with LC,105 patients with HCC under the background of the hepatitis B and 107 healthy controls.x2 test was used to test the Hardy-Weinberg equilibrium; genotype, allele and haplotype frequencies were compared between CHB group, LC group, HCC group and control group respectively by logstic regression adjusted for sex and age using the software SPSS 13.0. The haplotypes and analyzed was constructed by PLINK software.Results1. The differences of age and gender composition between CHB, LC, HCC group and control group were not statistically significant (P>0.05). Genotype of IL-2+114 and-384 in the other three groups and control group were consistent with Hardy-Weinberg equilibrium (.P>0.05)2. There were TT, TG and GG genotypes in IL-2+114G/T were found in all of 4 groups. The difference of the frequency distribution between the control group and the other groups were no statistically significant (P>0.05). Comparing with G allele, T allele was associated with susceptibility to CHB (P=0.012), so the T allele was associated with a significantly lower risk of CHB as compared with the G allele (OR=0.615,95%CI:0.421～0.897); T allele was also associated with susceptibility to LC(P=0.022), so the T allele was associated with a significantly lower risk of CHB as compared with the G allele (OR=0.615,95%CI:0.421-0.897)3. The polymorphisms of IL-2-384T/G were found in all of 4 groups, but genotype and allele frequencies in CHB,LC and HCC patients were not significantly different from those in control group respectively (P>0.05).4. In the male population, the presence of T allele of+114G/T was associated with susceptibility to LC (P=0.001); GG genotype and G allele of-384T/G was associated with susceptibility to LC (P=0.007, P=0.003). In the female population, the presence of T allele of+114G/T was associated with susceptibility to CHB (P=0.009); TG genotype and G allele of-384T/G was associated with susceptibility to LC(P0.010, P=0.021).5. The distribution of the two SNPs in the control group were similar with the findings from the Asian subjects, but is significantly different in Utah of United States and Nigeria population.6. The TT haplotype frequency in the CHB group was significantly lower than that in the control group(P=0.008).Conclution The+114G/T and-384T/G polymorphisms of IL-16 gene were found in all of 4 groups. The+114G/T was associated with susceptibility to CHB and LC, the risk of patients carrying T allele developing CHB and LC was significantly reduced; TT haplotypes were associated with susceptibility to CHB; The distribution of the two SNPs in the control group were similar with the findings from the Asian subjects, but is significantly different in Utah of United States and Nigeria population.