| Objective Endothelial lipase (EL) is a major determinant of high-density lipoprotein (HDL) metabolism, it takes part in the development of atherosclerosis and coronary heart disease. Endothelial lipase gene (LIPG) polymorphisms affect the activity of expression and plasma levels. Several previous studies have showed that LIPG 584C>T polymorphism was association with high-density lipoprotein-cholesterol (HDL-C) levels and susceptivity of coronary artery disease, but the results are conflicting in different populations. Bai Ku Yao (White-trouser Yao) is an isolated ethnic subgroup of the Yao minority in China. Little is known about the association of LIPG 584C>T polymorphism and serum lipid levels in this population. This study was designed to detect the association of LIPG 584C>T polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 645 subjects of Bai Ku Yao and 638 participants of Han Chinese aged 15-85 were randomly selected from our previous stratified randomized cluster samples. Epidemiological survey was carried out using internationally standardized methods. Information on demographic characteristics, socioeconomics status, cigarette smoking and alcohol consumption was collected with standardized questionnaires. Blood pressure, height, weight, serum lipid and apolipoprotein (Apo) levels were measured, and body index mass (BMI) was calculated as a measure of weight relative to height. Genotyping of the LIPG 584C>T was performed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) combined with gel electrophoresis, and then confirmed by direct sequencing. The frequencies of genotypes and alleles were calculated by gene counting.Results (1) The levels of serum total cholesterol (TC), HDL-C, low-density lipoprotein-cholesterol (LDL-C), ApoAI and ApoB were lower in Bai Ku Yao than in Han (P < 0.05-0.001). There was no significant difference in triglyceride (TG) levels and the ratio of ApoAI to ApoB between the both ethnic groups (P > 0.05 for each). (2) The frequencies of CC, CT and TT genotypes were 50.4%, 46.2% and 3.4% in Bai Ku Yao, and 41.4%, 53.1% and 5.5% in Han (P < 0.01); respectively. The frequencies of C and T were 73.5% and 26.5% in Bai Ku Yao, and 67.9% and 32.1% (P < 0.01); respectively. The frequency of T allele in Bai Ku Yao was lower than that in Han. (3) Serum HDL-C levels in both ethnic groups were different among the three genotypes (P < 0.05 for each). The levels of HDL-C in TT genotype were the highest among the three genotypes. Serum TC levels in CC genotype were lower than those in CT/TT genotypes in both ethnic groups (P < 0.05). The T allele carriers in Bai Ku Yao had higher serum HDL-C and ApoAI levels than the T allele noncarriers (P < 0.05). Serum TG levels in CC genotype was higher than those in CT/TT genotypes in Han (P < 0.05). No significant difference in the remaining lipid parameters was found among the three genotypes in both ethnic groups (P > 0.05 for all). (4) Multivariate logistic regression analysis showed that the levels of HDL-C and ApoB were correlated with genotypes in Bai Ku Yao (P < 0.05 for each), whereas the levels of TC and HDL-C were associated with genotypes in Han Chinese (P < 0.05 and P < 0.01). Serum lipid parameters included age, sex, weight, BMI, blood pressure, cigarette smoking and alcohol consumption were also correlated with several environmental factors in the both ethnic groups.Conclusions (1) There were significant differences in the genotypic and allelic frequencies of LIPG 584C>T polymorphism between Bai Ku Yao and Han populations, the frequency of LIPG 584T allele is lower in Bai Ku Yao than in Han Chinese. (2) The LIPG 584C>T polymorphism is associated with serum HDL-C, TC and ApoB levels. (3) The differences in serum lipid levels between the two ethnic groups might partly results from different genotypic and allelic frequencies of LIPG 584C>T or different LIPG-environmental interactions. |
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