To Observe Inhibition Of Interferon Combinate Fluorouracil For Proliferative Vitreoretinopathy (PVR).

Objective: To observe inhibition of interferon combinate fluorouracil for ProliferativeVitreoretinopathy (PVR).Methods:The 24 color rabbits were randomly divided into A, B, C, D four groups. Each group lefteye is experimental eye. vitreous injected plasma, which was full of platelets, to establishedanimal PVR model. 2 days after model, group A intravitreal injected 0.2ml balanced salt solution;group B intravitreal injected 0.2ml interferon-r, group C intravitreal injected 0.1ml 0.5mg offluorouracil and 0.1ml 104 interferon -r [32];group D intravitreal injected of 0.2ml 0.5mg offluorouracil. Test experimental eye by slit lamp, fundus examination everyday, and at 7,14,30day test respectively [14]by flash electroretinogram (F-ERG), retinal B-scan,Retinalhistopathology examination.Results:⑴Slit lamp examination: different check point in time, four groups of experimental anteriorsegment eye abnormalities were not obvious. After injection, A group of no vitreous opacity.Other groups were mild vitreous opacity, but with time are gradually reduce turbidity.⑵Fundus examination: PVR-level of the experimental groups were progress with time, butgroup C developed slower. At the same time of 7d,14d,21d( after inject), each group was nosignificant difference (P> 0.05); 30d, group C PVR level lower than the other groups, thedifferences among groups was statistically significant (P <0.05), groups B,D and A comparisonbetween groups was statistically significant (P <0.05), group B,D comparison showed nostatistically significant (P> 0.05).⑶F-ERG examination: Before modeling F-ERG test results: b wave amplitude average of148.00±6.89μV. 1 day after modeling, the amplitude difference of b wave among the groupswas not significant, but all amplitude is lower than normal volatility. After injection 14,30 d, theamplitude difference of group B,C,D had not significant (p> 0.05), but there were differencesbetween the groups with the A, and was statistically significant (p <0.05).⑷Eye B-ultrasound: 14d, group A vitreous was opacity, retinal had thick proliferationmembrane, and 2 eyes retinal had detach; B and D groups had varying degrees of vitreousproliferation cable, but no retinal detachment. Group C had no obvious abnormalities. In 30d ,Retinal of group A thicked obviouly, and had 5 eyes occurred retinal detachment; groupsB,D had varying degrees of vitreous opacity, retinal thickening, Group B had 2 eyes,group Dhad 3 retinal detachment. Group C was a little vitreous opacity, some proliferation of the cable,but no retinal detachment.⑸Retinal histopathology:Normal structure of rabbit retinal layers arranged in neat, clear,internal limiting membrane is continuous and smooth. 14d of after inject, retinal membranesurface of group A proliferated, and the layers of structure disordered; retinal of group B,Dthicked, but the floors were normal; retinal of group C basic did not change, layers structure wasnormal. After injection 30d, the retinal surface of group A formed fold, and have fibrovascularmembrane proliferated; retinal surface of group C had mild fiber proliferate; retina surface ofgroup B and D had thick fibrous proliferation.Conclusions:Vitreous injected 0.1ml 0.5mg FUunites 0.1ml 104interferon-r to treat proliferativevitreoretinopathy, its efficacy is superior to 0.2ml 0.5mg fluorouracil or 0.2ml104 interferon-r.