Effect Of Transformation Growth Factor Beta (TGF-β) On The Proliferation And BMP-2 And Cbfal MRNA Expression In Cultured Rat Osteoblast

ObjectiveTo study the effect of TGF-βon the proliferation and expression of BMP-2and Cbfal mRNA in primary cultured rat calvarial osteoblasts .And investigate the possible mechanism of aetion on osteoblasts, provide further theorctical basis for clinieal medieation.Methods1 Culture rat osteoblasts, and observe their morphology and function2 The rat ostcoblasts are cultured in vitro,the effect of different levels of TGF-β(0,0.01,100ng/ml)on Primary cultured rat osteoblasts proliferation is assessed by MTT colorimetry after 24 hours, and then detennine their ODmV.3 The osteoblasts are cultured with different dose of TGF-β(0,0.01,100ng/ml). .The expression of BMP-2 and Cbfal mRNA in Primary cultured rat osteoblasts is analyzed by semiquantative RT-PCR.4 Statistics analysis:Date are presented as x±s.All statistical analysis is performed with Windows SPSS 13.0. The groups are determined by LSD.Heterogeneity of variance, the groups are determined by Dunnett T3, P<0.05 difference was statistically significant.Results1 Most of Primary cultured rat osteoblasts were elongated spindle-shaped.ALP stainingwas positive. After adding glycerophosphate-βand ascorbic acid一L into the medium,the calcium alizarin red staining was positive.2 Different concentration conversion growth factor beta (0, 0.01, 100ng/ml) in the role of the original and cultivate osteoblast after 24 hours can promote its appreciation,OD value increased significantly P<0.05. TGF-βObviously accelerate osteogenesis cell proliferation..3 Through the semi-quantitative RT-PCR detection former generation training osteoblast BMP-2 and Cbfal mRNA expression . The results showed that transformation growth factor beta increase BMP - 2 and cbfalmRNA expression , a dose effect relation. Control group and experimental comparison, experimental comparison are statistically significant (P < 0.05) .Conclusions1 Conversion growth factor beta promote the original generation training osteogenesis cell proliferation and differentiation and mature . 2 Conversion growth factor beta by adjusting the BMP - 2 and Cbfal expression, so as to promote bone formation.