The Expression Of Maspin PCNA COX-2 Protein In Ovarian Neoplasm And The Effect Of Celecoxib In Ovarian Cancer Cell

Objective: (1) To investigate the expression of Maspin,PCNA and COX-2 protein of epithelial ovarian carcinoma, and their correlation with the clinicopathological features. (2) To explore the effect of celecoxib and DDP on growth and apoptosis of human ovarian cancer cell line HO-8910 and its mechanism by detecting the distribution of cell cycle ,the rate of apoptosis and the expression of Maspin,PCNA and COX-2 protein.Methods: (1) The expression of Maspin,PCNA and COX-2 protein was detected by immunohistochemistry technique in 36cases of ovarian serous cystadenocarcinoma, 12 cases of borderline serous cyst adenoma and 21cases of serous cyst adenoma. (2) Human ovarian cancer cell line HO-8910 were cultured and treated with celecoxib and/or DDP in vtro, respectively. Cell proliferation was detected by MTT method. (3) The distribution of cell cycle and the rate of apoptosis were determined by flow cytometry. (4) The expression of Maspin,COX-2 and PCNA protein was detected by immunohistochemistry.Results: (1) In borderline serous cyst adenoma and serous cyst adenoma, the positive rate of Maspin protein was significantly lower than those in ovarian serous cystadenocarcinoma (P<0.05). The positive rate of Maspin protein was higher in G3 phase than in Gl-G2 phase (P<0.05), and lower in stageâ… /â…¡than in stageâ…¢/â…£(P<0.05). While the positive expression of PCNA were same to that of Maspin. The expression of Maspin and PCNA showed no significant difference between the patients in different ages (P>0.05). The positive rate of PCNA protein was lower in G3 phase than in Gl-G2 phase (P<0.05). The expression of PCNA showed no significant difference between the patients in different stages(P>0.05). In ovarian serous cystadenocarcinoma, the expression of PCNA were positive to that of Maspin . the expression of COX-2 were positive to that of Maspin ,the expression of PCNA were positive to that of COX-2.In ovarian serous cystadenocarcinoma, COX-2 protein expression was higher than those in borderline serous cyst adenoma and serous cyst adenoma, but there were no significantly difference among the different ages and clinical stage(P>0.05). The positive rate of COX-2 protein was significantly lower in G3 phase than in Gl-G2 phase (P<0.05).(2) The proliferation of HO-8910 cells was inhibited of celecoxib or DDP. There was positive interaction of celecoxib and DDP on cell proliferation(P<0.01). (3) The cell cycle was arrested in G₀/G₁ phase and the apoptosis was inducted by either celecoxib or DDP alone (P<0.05). Combined use of celecoxib and DDP could increase the inhibitory effect and the apoptosis rate (P<0.05). (4) Celecoxib or DDP alone could down-regulate the Maspin,PCNA and COX-2 protein expression (P<0.05). Combined use of celecoxib and DDP, the Maspin,PCNA and COX-2 protein expression was enhanced(P<0.05).Conclusion: (1) The expression of Maspin,PCNA and COX-2 protein in ovarian serous cystadenocarcinoma increased. The expression of Maspin was negatively correlated with clinical stage. The expression of PCNA was positively correlated with histological grades of the tumors and clinical stage. The expression of COX-2 protein was positively correlated with histological grades of the tumors. (2) Both celecoxib and DDP can inhibit the proliferation of HO-8910 cells. Celecoxib can enhance the inhibitory effect of DDP on HO-8910 cells proliferation. (3)Celecoxib and DDP can down-regulate Maspin,PCNA and Cox-2 protein expression and induce G0/G1 arrest of HO-8910 cells.