New Nano-hydroxyapatite / Deproteinized Cancellous Bone / I Collagen Combined Bio Degradable Porous Scaffolds And Repairing Bone Defects In Rabbits

Objective: To explore the new nano-hydroxyapatite / deproteinized cancellous bone / I collagen (nHA-DPB/COL I) complex biological porous biodegradable scaffolds methods, and preliminary study of its biological properties. Of nano hydroxyapatite / deproteinized cancellous bone / I collagen complex biological porous biodegradable scaffold Discontinued rabbit radius defect effects, and to observe the biodegradation period, and as bioactive factors and antibiotic Delivery carrier, to guide in the bone, conduction, induction and other aspects of bone regeneration. Scaffold for new research to provide new ideas and methods.Method: 1. Cancellous bone from the femoral part of the pig drawn by the ultra-low temperature freezing and removal of protein, the preparation of deproteinized cancellous bone;2. Preparation by impregnation nHA-DPB/COLI new compound bio-degradable porous scaffold, according to the method of biomimetic synthesis of nano hydroxyapatite / I collagen scaffold; 3. Choose 36 New Zealand rabbits made of 15mm radius bone defects were randomly divided into 4 groups, A group implanted nHA-DPB/COL I combined bio degradable porous scaffold, set to the experimental group, B, C, D groups were deproteinized cancellous bone, autologous bone and nano-hydroxyapatite / I collagen composite scaffold,, is set to experimental control group. 4,8,12 weeks after surgery animals were killed, each batch of each group were sacrificed 3, for the following tests: general observation, X-ray examination, X-ray image analysis, scanning electron microscopy, histological observation, tissue sections Repair of computer image analysis measurements of new bone area, bio-mechanical testing and results were statistically analyzed.Results: 1, nHA-DPB/COL I combined bio degradable porous scaffold pore size, porosity and compressive strength was 150 ~ 300μm, 62.59% ~ 79.36%, 27MPa ~ 53MPa; 2, 4,8,12, respectively Week by histological examination and X-ray examination showed the extent of bone defect, proof of the experimental group and autograft bone was no significant difference, better than the nano-hydroxyapatite / I collagen composite stent group and deproteinized cancellous bone Group; 12 weeks after biomechanical testing showed that the experimental group compared with normal bone strength, anti-torsion test no significant difference. Conclusion: Prepared by biomimetic deposition of new nHA-DPB/COL I Compound biological porous biodegradable stent body with no rejection, no allergic reactions, have good biocompatibility, bone conduction, can efficiently induce the degradation and bone formation activity. nHA-DPB/COL I combined bio degradable porous scaffold is a very good bone repair materials, as tissue engineering scaffold material has its advantages.