Effects Of Sufentanil Post-conditioning On Acute Lung Injury Induced Ischemia-reperfusion In Rats

Objective Research sufentanil on intestinal ischemia-reperfusion rat lung tissue and serum tumor necrosis factor-α(TNF-α) and lung tissue nuclear factor-κB (NF-κB), and investigate the effects of Sufentanil post-conditioning (SP) acute lung injury induced by ischemia-reperfusion in rats and possible mechanisms, In order to provide experimental basis for clinical.Methods Thirty-two healthy male Wistar rats were randomly divided into four group (n=8): Sham-operation group (S group),I/R group(I/R group),intestinal ischemic Post-conditioning group(I-post group) and 1ug/kg Sufentanil post-conditioning group (SP group).Each group of 8.(1)S group :Superrior mesenteric artery was only isolated but not blocked.(2)I/R group was established by clamping superior mesenteric artery(SMA)for 1 hour and reperfusing for 2 hours.(3) Line immediately after 60 min ischemia and 3 cycles of reperfusion 30 s / block 30 s, more than with the I / R group. (4) The 1ug/kg sufentanil diluted to 0. 3ml immediately after reperfusion through the femoral vein with a syringe pump pump 0.1ml/30S, during the intermittent 30s, so the cycle 3 times (a total of 0.3ml), than with the I / R Group. The animals were killed at 2 h of reperfusion respectively (n=8). HE dyed observe lung tissue pathology changes in rats. TNF-αin the serum and lung tissue were determined using radio-immunifaction method .Expression of NF-κB P65 in the lung tissue was assessed by immunohistichemical method.Results (1) HE detection pathological changes:①S group: not found significant pathological changes, alveolar size uniform, complete structure, wall thin, did not see the bleeding edema and inflammatory cells infiltrating;②I/R group visible alveolar size is uneven and integrity damage, alveolar exudate of cavity have protein samples, the alveolar wall thickening widened, the interval, interstitial lung and alveolar edema, a lot of inflammatory cell, congestion, bleeding obvious.③I-post group and SP group are lighter the pathological changes, the alveolar lumen protein, the alveolar exudate is reduce thickened wall, inflammatory cell ease.Compared with the sham-operated control group, serum and lung TNF-a lever was increased significantly in the other groups ( ), and NF-kB p65 protein in the lung was increased (P<0.05). Compared with the I/R group, TNF-αin the serum and lung tissues were decreased significantly following Sulfentanil treatment ( ), and the expression of lung NF-κB was markedly ameliorated (P<0.05).There were no significant difference between I-post group and SP group( ). Conclusion (1)This study demonstrated that intestinal ischemia-reperfusion l may result in sincerely lung damage.(2) The mechanism of lung injury induced by intestinal ischemia-reperfusion is complicated, and its occurrence and development may be related to TNF-αand NF-KB related.(3) sulfentanil post-conditioning on intestinal ischemia-reperfusion induced lung injury has a protective effect, can inhibit NF-κB in lung tissue expression of rat intestinal ischemia and reperfusion reduce the serum and lung tissue content of TNF-α.