Exploring The Pathogenesis Of The Systemic Lupus Erythematosus By TLR2, TLR4 And HMGB1

Objective: By detecting the expression of HMGB1 in Serum, TLR2 and TLR4 in monocyte derived macrophage in Systemic Lupus Erythematosus Patients, to evaluate the pathogenesis and the role of disease progression in SLE.Methods: According to the American College of Rheumatology (ACR) 1997 in SLE:To select 40 patients with systemic lupus erythematosus 6 males and 34 females, Aged 13 to 70 years, mean age (33±14) years and 20 healthy volunteers 3 males and 17 females, aged 18 to 61 years, mean age(34±12) years which did not occur infection within one month recently. Each patient were recorded clinical symptoms and serological examination of the indicators, test scores according to the SLEDAI-2000. The 40 patients were divided into activity groups (≥10 points) and the stable group (<10 points) according to the results of test scores, each group was 20 patients.The expression of HMGB1 in serum of these cases were detected by ELISA; The expression of TLR2 and TLR4 on CD14+ monocytes in peripheral blood were detected by FCM; and meantime the complement C4, ds-DNA, anti-Clq antibody, anti-nucleosome antibodies in peripheral blood were detected; to analyze the correlation between these indexes and clinical, laboratory indexes about SLE using statistical methods.Results: The expression levels of HMGB-1 in serum of the active SLE group compared with the stable and normal control group was significantly higher (P<0.05). But the serum HMGB-1 expression levels in the stable SLE and normal control group were not significantly different (P>0.05). There were positive correlation between the active SLE group,s serum levels of HMGB1 and Anti-Clq antibodies, anti-nucleosome antibodies, ds-DNA (P<0.05), negative correlation and the complement (P<0.05). The expression of TLR2 and TLR4 in peripheral blood mononuclear cells derived macrophages in the active SLE group compared with the stable and normal control group was up-regulated, but the expression of TLR2 and TLR4 between the stable and normal control group were not significantly different (P>0.05). There were positive correlation between the serum levels of HMGB1 and TLR2 and TLR4 in peripheral blood mononuclear cells derived macrophages (P<0.05).Conclusion: The expression levels of HMGB-1 in serum and the expression of TLR2 and TLR4 in peripheral blood mononuclear cells derived macrophages all participated in the pathological processes of SLE. The expression of HMGB1 and TLR2/TLR4 in SLE were elevated and they were associated with the occurrence and development of SLE. Meanwhile, there were positive correlated between the serum levels of HMGB1 and anti-nucleosome antibodies, anti-ds-DNA antibodies, so it was suggested that HMGB1 may be correlate with the abnormal immune states of the body. The expression of HMGB1 and TLR2/TLR4 may be synchronous in the pathogenesis of SLE.