| Abstract: Objective: to investigate the association of MMP-9 gene P574R, R668Q polymorphism in population in Lu Zhou area of Sichuan Province of China and susceptibility of colorectal cancer, clinic tumor makers of colorectal cancer, clinical pathology parameters, clinical biochemical indicators of lipid. Methods: randomly selected 228 cases of colorectal cancer patients and 100 healthy population in Sichuan Luzhou Medical College Hospital diagnosed in 2009-2011, removed Venous blood from body, extracted genomic DNA from blood using method of phenol-chloroform-isoamyl alcoho, amplified Specific genes using PCR. Techniques of restriction enzyme digestion, Polyacrylamide gel electrophoresis and silver staining were used to identify MMP-9 P574R, R668Q locus. Detailed records of tumor markers of clinical detection of in (AFP, CEA, CA19-9, CA72-4), pathological parameters (site , gross type, histological type, differentiation, invasion depth, lymph node metastasis), biochemical parameters and lipid biochemical parameters (TC, LDL-C) newly diagnosed colorectal cancer patients were needed. SPSS13.0 software were used to analyze genotype, tumor clinical parameters, using chi-square test.?clinical detection of tumor markers, clinical and biochemical indicators of lipid were used by T test. P < 0.05 statistically significant, the relative risk odds ratio (odds ratio, OR) values and 95% confidence interval (95% confidence interval, 95% CI), were used to express gene Type and allele and colorectal cancer relevance. Results:? 1. MMP-9 P574R and R668Q locus genotype frequencies between Patient group and healthy group were consistent with Hardy-Weinberg equilibrium. P574R and R668Q genotype frequencies in the two cases - the distribution of the control group there was no significant difference between the two genotypes and no correlation between the incidence of colorectal cancer (P = 0.481, OR = 0.653, 95% CI = 0.234 -1.823; P = 0.073, OR = 3.574, 95% CI = 0.986-12.958); MMP-9 P574R allele locus P, R allele frequency between patient group and healthy group, is of no significant differences in the distribution [P=0.490; OR=0.624; 95% CI=0.228-1.705]; MMP-9 R668Q locus R allele, Q allele frequency between patient group and healthy group, is of no significant differences in the distribution [P = 0.075; OR = 3.495 ; 95% CI = 0.975-12.523]. 2. AFP of colorectal cancer patients in the MMP-9 P574R sites PP, PR + RR genotype was no of significant difference (P = 0.205); CEA value of colorectal cancer patients in the MMP-9 P574R sites PP, PR + RR genotype is of no significant difference (P=0.745); CA19-9 of colorectal cancer patients in the MMP-9 P574R sites PP, PR + RR genotype was of no significant difference (P=0.566); CA72-4 of colorectal cancer patients in the MMP-9 P574R sites PP, PR + RR genotype was of no significant difference (P=0.460) .3. The frequency of the 574PP, PR + RR genotype loci in the Colorectal cancer was not significantly different in different tumor location (colon, rectum) (P=0.752, bilateral); The frequency of the 574PP, PR + RR genotype loci in the Colorectal cancer was not significantly different in different tumor gross type (ulcer-type, bulge-type) (P = 1.000, bilateral); The frequency of the 574PP, PR + RR genotype loci in the Colorectal cancer was not significantly different in different tumor size (≥3 cm, <3 cm) (P = 0.713, bilateral); The frequency of the 574PP, PR + RR genotype loci in the Colorectal cancer was not significantly different in different histological types (adenocarcinoma, mucinous carcinoma) was not significantly different (P = 0.638, bilateral); The frequency of the 574PP, PR + RR genotype loci in the Colorectal cancer was not significantly different in different tumor differentiation (well?differentiated+ well-middle differentiated, middle differentiated+poorly?differentiated) was not significantly different (P = 0.749, bilateral); The frequency of the 574PP, PR + RR genotype loci in the Colorectal cancer was not significantly different in different invasion depth (not yet reached full thickness, full thickness) was not significantly different (P = 1.000, bilateral); The frequency of the 574PP, PR + RR genotype loci in the Colorectal cancer was not significantly different in different lymph node metastasis (no metastasis, metastasis) was not significantly different (P = 0.766, bilateral).4. the difference of TC between P574R PP and PR+RR genotype in Colorectal cancer was statistically significant (P = 0.038); TC value of PR or RR genotype in patients with colorectal cancer is lower than the value of the PP; The difference of LDL-C between 574PP and PR + RR genotype in Colorectal cancer was statistically significant (P = 0.043, bilateral), LDL-C values of PR or RR genotype in patients with colorectal cancer is lower than PP genotype. Conclusion: 1. MMP-9 P574R, R668Q polymorphism has nothing to do with susceptibility to colorectal cancer; 2. MMP-9 P574R polymorphism has nothing to do with biochemical indicators of colorectal cancer (AFP, CEA, CA19-9,CA72-4).3. MMP-9 P574R polymorphism and clinicopathological parameters (site, gross type, histological type, differentiation, invasion depth, lymph node metastasis) in colorectal cancer are independent. 4. MMP-9P574R polymorphism in patients with colorectal cancer has something to do with clinical biochemical indicators of lipid. And MMP-9 P574R polymorphism in patients with colorectal cancer may be a TC and LDL-C level of risk factor levels. |
