A Metabonomics Study About Liver Cirrhosis And Liver Cancer Plasma Based On UPLC/Q-TOF-MS

Objective: We used the metabonomic method to analyze the different metabolic substances in the sera of liver cancer which induced by HBV and HCV respectively .Different metabolic substances in the sera of liver cirrhosis , liver cancer and normal persons, and finally find potential tumor biomarkers.Methods: UPLC/Q-TOF-MS was utilized to analyse the different level metabolic substances in sera among 29 cases of liver cirrhosis which induced by HBV and HCV respectively , 38 and 32 cases of liver cancer sera which induced by HBV and HCV respectively ,and 30 cases of normal persons .We got retention time ,m/z and total ion strength related figure ,then matched peaks, normalized these peaks ,and analysed the data with PCA and PLS-DA models, matched the related substances in human metabonomics database and confirm this substances by standard substance.Results: The patients of HBV and HCV induced liver cancer can not be separated in the score plots of PCA and PLS-DA models .Twenty-six substances were identified as potential markers, ten compounds were identified as: LysoPC(20:1) , LysoPC(20:2) , LysoPC(P-18:0) , LysoPC(P-18:0) , LysoPC(15:0) , arachidonic acid , hemolysis phosphatidyl choline , carnitine, glycine, tryptophan, N-Arachidonoyl glycine , which as the potential tumorbiomarkers of liver cancer . Conclusion:1.There were no differences between patients of HBV and HCV induced liver cancer in metabolites level.2.Through the analysis between liver cirrhosis and liver cancer , Twenty-six different substances were found as potential tumor biomarkers, LysoPC(20:1) , LysoPC(20:2) , LysoPC(P-18:0) , LysoPC(P-18:0) , LysoPC(15:0) ,arachidonic acid , hemolysis phosphatidyl choline , carnitine(C18:1), carnitine(C18:2), glycine, tryptophan, N-Arachidonoyl glycine , which as the potential tumor biomarkers of liver cancer .