| [Objective] To observe the expression of NF-κB in the external auditory canal cholesteatoma, middle ear cholesteatoma and middle ear granulation tissue. To preliminary discuss the relationship between NF-κB and bone destruction of middle ear cholesteatoma, illuminate the pathogenesis of middle ear cholesteatoma.[Method] To collect 10 cases of external auditory canal cholesteatoma,20 cases of middle ear cholesteatoma,20 cases of middle ear granulataion tissue,10 cases of normal external ear canal skin. To detect the expression of NF-κB in the above tissue through the immunohistochemical MaxvisionTM2.[Result]1. The expressions of NF-κB were positive in the 20 cases of middle ear cholesteatoma,14 cases were strongly positive. The NF-κB expressions were distributed in all epithelial layers, which were most located in all basal lamina and prickle cell layer, some were located in inflammatory cells and vascular epithelial. They were negative or weakly positive expression in keratosis of cholesteatoma.12 cases were positive in nucleolus.2. The expressions of NF-κB were positive in the 20 cases of middle ear granulation tissue,5 cases were positive in nucleolus.3. The expressions of NF-κB were positive in 10 cases of external auditory canal cholesteatoma,2 cases were positive in nucleolus.4. The expressions of NF-κB were negative or weakly positive in cytoplasm of normal external ear canal skin, it didn't appear the phenomenon of nuclear translocation.[Conclution]1. Comparing with the normal skin, the expressions of NF-κB were significantly higher in cholesteatoma and granulation tissue. 2. Statistical correlations were established between the expressions of NF-κB in middle ear cholesteatoma and the degree of bone destruction, indicating that the high expression of inflammatory factors because of the upregulated NF-κB maybe one of the factors leading to bone destruction.
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