The Expression And Related Research Of Tumor Necrosis Factor-α And Matrix Metallo-pteinase-9 In Cholesteatoma And Bone Destruction

Objective:1 Discussion tumor necrosis factor - Alpha,matrix metal proteinase - 9 in middle ear cholesteatoma expression situation and expounds its reciprocity.2 Discussion necrosis factor - Alpha,matrix metal proteinase - 9 and patient ear bone and periphery ossein destructiveness relations.Method:Specimen from Shanxi Medical University first clinical hospital otolaryngology,in collection cholesteatoma tympanitis patient surgery fresh specimen,rapid fixed and maked into the paraffin section.At the same time the extent of bone destruction was noted.Uses the immunohistochemical technology and the computer image analytic method was used to examine the expression of tumor necrosis factor-alpha,matrix metal proteinase-9 in 23 example cholesteatoma's expression,simultaneously observes the correlation between the expression of and the bone destroys reciprocity and takes the comparison by 10 external ear canal skin.The aim to the elucidate mechanism of bone destruction.Result:1 TNF-α,the MMP-9 protein immunohistochemical staining positive for result is the brown granular distribution.The both mainly locate in cytoblastema.TNF-αexpression under cholesteatoma stomal cell and epidermis.MMP-9 expression in cholesteatoma stromal cell.2 In the cholesteatoma and the external ear canal skin,the average optical density value of TNF-αwas 0.531±0.2331 and 0.073±0.06.7,there was statistical significance among the two groups(P=0.000);the average optical density value of MMP-9 was 0.4833±0.2659,0.0926±0.0688,there was statistical significance among the two groups(P=0.000);in addition,by relation analyse we found that over expression of MMP-9 is significanty associated with the expression of TNF-α(r=0.843,p=0.00).3 TNF-αdemonstrates four levels in the cholesteatoma to bone destruction labeling index expression average after the statistical analysis Between the average the difference has statistics significance(P＜0.05),Further makes comparison discovery different rank between the difference has statistics significance,indicated that TNF-αis relative to the bone destruction.4 MMP-9 in the cholesteatoma to bone destruction labeling index graduation expression average after the statistical analysis demonstrated that between four levels of average the difference has statistics significance(P＜0.05),Further makes comparison discovery different rank between the difference has statistics significance,indicated that MMP-9 is relative to the bone destruction.Conclusion:1 TNF-αin the cholesteatoma high expression,increases along with TNF-αthe expression quantity,the bone destructiveness also along with the aggravation,indicated that it closely relative to the bone destruction.2 MMP-9 in the cholesteatoma high expression,increases along with the MMP-9 expression quantity,the bone destructiveness also along with the aggravation,indicated that it closely relative to the bone destruction.3 TNF-α,MMP-9 expression has the relevance,prompts between the two to have the interaction,the latter is possibly the former's induction factor.4 Over expression of MMP-9 only are observed in stomal cells,the immostain of TNF-αalso are observed in stomal cells indicating that stomal cell play an important role in bone destruction.5 In the cholesteatoma TNF-α,the MMP-9 high expression concerns these target examination in the bone destructiveness to be possible for clinical on to determine that the surgery way, the appraisal prognosis provide the reference.