Study On The Antioxidant Activity And Anticancer Activity Of Selenium Nanoparticles Sol

The biological effects of selenium nanoparticles (nanoSe0) were multiple. The study of this subject was about the antioxidant and the anticancer activity of nanoSe0 sol.The antioxidant capacity of nanoSe0-amino acid sols were evaluated by using chemical oxidation model in vitro, such as, Fenton system, pyrogallol autooxidation system, DPPH assay, and the oxygen radical absorbance capacity (ORAC) assay. The experimental results demonstrated that nanoSe0-amino acid sols could scavenge the hydroxyl radical (OH-·), superoxide anion free radical (O2-·), and DPPH radical efficiently, and the scavenging capacity for all kinds of radical were different from each other. In addition, the results of ORAC showed that the total antioxidant capacity of the nanoSe0-amino acid sols were linearly related to its concentration. So nanoSe0-amino acid sols could be used as natural antioxidant component.The accumulated H2O2 could result in lipid peroxidation, RBCs auto-haemolysis. While nanoSe0 sol was used as a preprotective agent, the results indicated that nanoSe0 sol displayed a significant inhibition of RBCs haemolysis induced by H2O2. Furthermore, these results validated that the accumulated OH-·can result in cell membrane lipid oxidation. However, RBCs treated with nanoSe0 sol were protected from lipid oxidation by using atomic force microscope (AFM). So, nanoSe0 sol could protect the RBCs oxidative damage induced by H2O2 and OH-·in cell system and possesses antioxidant activity.nanoSe0 sol (10～40μmol/L) exhibited the intense killing effect on the human cervical carcinoma (HeLa cells) and human breast carcinoma (MDA-MB-231 cells) by optical microscopic inspection and MTT assay, and that the nanoSe0 sol were able to kill HeLa cells and MDA-MB-231 cells in a dose-dependent way. The morphological features of HeLa cells treated with 10μmol/L nanoSe0 sol were significantly changed, and they had displayed rough and shrunken and so on. The flow cytometry (FCM) investigation demonstrated that low doses of nanoSe0 sol (10μmol/L) arrested cells in the M phase of the HeLa cell cycle, and inhibited of HeLa cells to proliferate and grow. The investigation demonstrated that nanoSe0 sol exhibit the intense killing effect on cancer cells, and inhibit of cancer cells to proliferate and grow. However, the result of AFM research indicated that the 41.4μmol/L Vc had no anticancer function. So nanoSe0 sol could be used as effective anticancer drugs.In conclusion, nanoSe0 could scavenge OH-·,O2-·and DPPH radical efficiently and could protect the RBCs against oxidative damage to maintain their physiological functions. Furthermore, nanoSe0 could inhibit the proliferation and growth of cancer cells.