The Study Of Curing Spinal Cord Injury In Rats By Transplantation Of Neural Stem Cells

Objective:To investigate within 24 hours of neonatal rat neural stem cells (NSCS) transplantation in rats with spinal cord injury repair neurological function.Methods:The NSCs were isolated from brain of rats, in vitro and identified. Production of spinal cord injury (SCI) model of spinal cord injury in the zone will be a good marker BrdU 7 days after injury for transplantation, spinal cord injury model is divided into three groups:A, injury, natural recovery after spinal cord injury; B group, NS transplantation group; C group, neural stem cell transplantation, spinal cord injury 7 days. ABC three groups of injury before and after injury,6 days,14 days,21 days,30 days,60 days were performed hindlimb BBB behavior score, injuries 60 days after experimental animals were killed for pathological examination and immunohistochemical staining was observed in rats transplanted NSCS survival, migration and differentiation.Results:1.24 hours of neonatal rat hippocampus in vitro in the NSCS can be isolated, cultured and passaged, nestin staining confirmed that the NSCS2. BBB function score:There were not significant differences among three groups Days after injury (P>0.05).Behavioral score of A group was significantly higher Than those of B and C group (P<0.05)and there were not significant difference Between B and C group(P>0.05)14 days after injury. Behavioral score in both A and C group was higher than that in B group (P<0.05)and there were Significant different between A and C group(P>0.05)21 days after injury. The Behavioral score in C group was the highest, in A group second and in B group Was the lowest(P<0.05)30 days after injury, as well as 60 days after injury. It was show that the behavioral score of model rats was significantly improved3. Pathology and immunohistochemical double stain:BrdU-Positive cells could be detected in the spinal cord of rats in C group. It was shown that NSCs could migrate in vivo. BrdU+NSE and BrdU+GFAP double-Positive cells could be observed in the injured spinal cord of rats in C group. It was suggested that NSCs could differentiate into neurons and astrocytes in vivo.Conclusion:The NSCs derived from the nervous system of the rats can be isolated, cultured and reproduced in vitro, The NSCs transplanted into the injured spinal cord injury of rats can survive, Neural stem cells into spinal cord injury the body can survive, differentiate into neurons and glial cells.