Effect Of Leonurine Infusion Before Ischemic Preconditioning On Myocardial Reperfusion Injury In Rat

Objective To investigate the cardioprotective effect of leonurine infusion before ischemic preconditioning on myocardial reperfusion injury in rat heart. Methods Isolated perfused working heart model were performed.40 hearts of rat were randomly divided into four groups, all hearts were subjected to 2-hour global hypothermic ischemia and received intermittent cold cardioplegia perfusion. Results During reperfusion,the recovery of left ventricular systolic pressure,left ventricular end diastolic pressure, +dp/dtmax ,and -dp/dtmax of hearts received leonurine infusion before ischemic preconditioning were significantly improved,myocardial superoxide dismutase activity were higher,the malondialdehyde content were lower,and myocardial CytC,Caspase-9,Caspase-3 expression were lower,and myocardial water content was obviously less.Conclusion These results suggest leonurine infusion before ischemic preconditioning enhances cardioprotection of ischemic preconditioning against myocardial reperfusion injury in the rat heart.