Expression Of Wnt-1,β-catenin And E-cadherin In Cervicel Carcinoma And Its Clinical Significance

Objective:To explore the role of Wnt-1,β-catenin and E-cadherin in the genesis and progression of cervical cancer by detecting the expression of Wnt-1,β-catenin and E-cadherin and to study the relationship between them. Material and Methods:Immunohistoehemistry SP method was used to detect the expression of Wnt-1,β-catenin, E-cadherin in 30 cases of nomal cervical epitheliun(NCE), 30 cases of cervical intraepithelial neoplasia(CIN) and 60 cases of cervical squamous cell carcinoma(SCC). The relationship of the expression of Wnt-1,β-catenin, E-cadherin and clinical pathological feature was analysed, and the correlation of the expression of Wnt-1,β-catenin, E-cadherin was explored.Results:1. The positive expression of Wnt-1 was located in cytoplasm.The positive expression rate of Wnt-1 in NCE, CIN and SCC was 20%, 66.6%, 88.3% respectively,and its positive expression rate was increased gradually based on the severity of the lesion from NCE to SCC. There was significant difference between the positive rate of them(P<0.001), and there was no significant relationship between the expression of Wnt-1 and clinical pathological feature such as age, tumor size, differentiation (p>0.05).2. The expression ofβ-catenin was mainly located in cell membrane of NCE,when the expression was decreased and located in the cytoplasm/nucleui in CIN and SCC. The abnormal expression rate ofβ-catenin expression in the cytoplasm/nucleui or absence in cell membrace of NCE,CIN and SCC was 10%, 53.3% and 71.7%, respectively, and there was significant difference among them(P<0.001). The expression ofβ-catenin was significantly associated with clinical stage (P<0.05) and the depth of invasion (P<0.05).3. The expression of E-cadherin was located in cell membrane. The positive expression rate of E-cadherin in NCE, CIN and SCC was 96.8%, 46.7%, 25% respectively. The positive expression rate of E-cadherin was decreased based on the severity of the lesion from NCE to SCC, and there was significant difference among them(P<0.001). The expression of E-cadherin was significantly associated with tumor differention(P<0.05), clinical stages(P<0.05), invasion (P<0.01) and pelvic lymphnode metastasis(P<0.05).4. The relationship of the expression of Wnt-1,β-catenin, E-cadherin in SCC There was significant positive correlation between the expression of Wnt-1 andβ-catenin (r=0.463,P <0.001), and there was significant negative correltion between the expression of E-cadherin andβ-catenin(r=0.090,P >0.05).No significant correlation was observed between the expession of Wnt-1 and E-cadherin(r=0.090,p>0.05).Conclusion1. From NCE, CIN to SCC,the positive expression of Wnt-1 and theβ-catenin level is increased gradually, which implys that they may play a role in carcinogenesis and development of cervical squamous cell carcinoma.2. From NCE, CIN to SCC, the positive expression rates of E-Cadherin is increased gradually, which may have ceorrelation with carcinogenesis and development of cervical squamous cell carcinoma.3. The abnormal expression ofβ-catenin is associated with clinical stages and invasion .And the decrease of expression of E-cadherin is associated with clinical stages, tumor differention, invasion and pelvic lymphnode metastasis. Both of them implys the severity of malignancy in SCC and may play a role in the invasionand metastasis of SCC . 4. There is signifieant positive ceorrelation between the expression of Wnt-1and β-catenin. It indicates that Wnt signaling pathway may be actived by Wnt-1, results to the accumulation ofβ-catenin in cell cytoplasm.5. There is signifieant negative ceorrelation between the expression ofβ-catenin and E-cadherin. The loss of E-cadherin promote the abnomal expression ofβ-catenin , and lead to the occurrence and development of SCC.