Effect Of Dihydrostestosterone On Synaptophysin And NMDAR Of Hippocampus In SAMP8

Objective:Observe the effect castration and androgen replacement therapy on hippocampal synaptophysin and NMDAR in senescence accelerated mouse-prone/8(SAMP8).Methods:Fifteen 6-month-old male SAMP8 were randomly divided into sham-operation control group(P8 group), castrated group, and androgen repla- cement therapy with castrated group(DHT group). Choose five 6-month-old male SAMR1 as normal contrast of the consanguinity group(R1 group). Castr- ated group and DHT group were removed twin testis. DHT group, hypodermic injection 1mg/(kg.day) dihydrotestosterone(DHT) after 1 day. Other groups were injected equal asepsis medical maize oil, 21days altogether.Cut heads of each group, got brain and dissected hippocampus. Using reverse transcription real-time quantitative PCR to research the expression changes of hippocampal synaptophysin and NR1, NR2A, NR2B.Results:1 The synaptophysin mRNA expression: Synaptophysin of R1 group was the strongest, for 1±0.23(P < 0.01); the expression of P8 group and DHT group were similar, for 0.84±0.21, 0.25±0.80, compared not statistically significant difference (P > 0.05). Castration groups express the most weak, for 0.56±0.17, less than other group (P < 0.05).2 NMDA receptors NR1, NR2A, NR2B hushais mRNA expression: R1 groups all have expressed the strongest(P < 0.01), for 1±0.34, 1±0.27, 1±0.28. NR1, NR2A, NR2B of P8 group and DHT group were similar, for 0.76±0.18, 0.81±0.24, 0.73±0.23; 0.73±0.22, 0.79±0.25, 0.83±0.22. Castration group express the most weak, for 0.59±0.15, 0.61±0.19, 0.55±0.17. (P < 0.05).Conclusion:1 SAMP8 mice as the ideal senile dementia research model, its memory cognitive function defects and cultured hippocanlpal neurons synaptophysin and NMDA receptors on expression is reduced.2 SAMP8 mice, its androgen deficiency after castration can further make synaptophysin and NMDA receptors expression is reduced. Androgen can certain degree increase SAMP8 mice cultured hippocanlpal neurons castrated synaptophysin and NMDA receptors expression.