Effects Of Early Exercise Intervention On Expression Of Synaptophysin In Hippocampus Of Cerebral Ischemia And Hypoxia Rats

Objective To observe the effects of early movement training on the expression of synaptophysin protein and Nissl body in hippocampus region of the neonatal rats’ brain after hypoxic-ischemic brain damage(HIBD), to investigate the possible mechanism of early movement training in recovery neuronal function after hypoxic-ischemic brain damage(HIBD) in the neonatal rat.Methods To establish animal model of hypoxic-ischemic brain damage in neonatal wistar rats. A total of 35 neonatal rats aged 7days were randomly divided into training group, control group, sham-operation group.For the training group, swimming 10 minutes a day for two weeks after hypoxicischemic brain damage 7days later; the control group did no training after hypoxic-ischemic brain damage;sham-operation group were subject to no HIBD and no swimming training. Western Blot was adopted to detect the expression of synaptophysin in the hippocampus of the brain;Nissl staining was adopted to observe neuronal cells in the hippocampus of the brain.Results 1.The expression of synaptophysin in the sinistrocerebral hippocampus of control group rats(0.447±0.141) were significantly lower than that in sham-operation group(1.027±0.035)(P <0.01). 2.The expression of Synaptophysin in the sinistrocerebral hippocampus of training group(0.772±0.121) was higer than that of the control group(0.447±0.141)(P<0.05).3.Nissl’s staining shows that there are more Nissl body in sham-operation group than control group,and there are more Nissl body in training group than control group.Conclusion Early movement training can increase the expression of synaptophysin and increase the number of Nissl body in the hippocampus of the rats’ brain after hypoxic-ischemic brain damage.