| ObjectiveEpidermal stem cells can be used as one of the elect when find the seed cells of tissue engineered skin because of their unlimited proliferation and the ability of differentiating into cells required in certain induced conditions. In this study, we use selected epidermal stem cells react with the inorganic active elements scaffold material. And the back wounds in 46 SD rats are treated by the scaffold material. What we do is to explore the biological induction of the scaffold for the epithelial cell.Methods(1) Cellular test: Select 8 cases of normal skin discarded after surgery organization from children with cleft lip (2-5years).Use typeâ…£collagen adhesion method to isolate epidermal stem cells (ESC), and then divided into two groups. The inorganic scaffold active element is added into experimental group (A group), and the other group (B group) is the control one. Then culture and identify the sorting cells. The cell proliferation rate was determined in 11 and 21 post culture days. And also the PH level were determined in 1d, 4d, 7d , 10d, 12d, 15d, 18d, 20d post culture days. (2) Animal test: Self-consubstantiality control was employed in the study. 40 SD rats were inflicted with two radius of 0.5cm(C group ) and 1.5cm(D group), depth of 0.15 ~ 0.25mm on the back of each rat, divided into treatment group(with scaffold material 1g/100cm2 topical application to the wounds) and control group(with topical application of erythrocin cream to the wounds). Observed the pathological changes of the wounds and calculated the wound healing rate in 3, 5, 7, 10, 13, 18d after treatment. Results1. Cellular test:(1)Epidermal stem cell were selected by means of typeâ…£collagen at different concentration(0.1mg/ml and 0.2mg/ml) in different time periods(10min,20min,30min). In 20 minutes group used 0.2mg/ml typeâ…£collagen, epidermal stem cell had better proliferation and differentiation after passage.(2) PH in the supernatant was alkaline after the first day, but gradually decreased from 4-20 days. (3)The cell proliferation rate in the culture supernatant were obviously higher than thosein control group at the same time points (P<0. 01).2. Animal test: For the wounds of two different sizes, hyper plastic granulation tissue or crawling speed of epithelial of the treatment group were significantly early than the control group of the same radius. The healing rate of smaller thickness wounds(R=0.5cm) in 7, 10, 13d, and that of bigger thickness wounds(R=1.5cm) in 5, 10, 18d were obviously higher than that in the control group of the same size wounds (P<0. 05).ConclusionAs one of the seed cells for skin tissue engineering, epidermal stem cells has great potential for the proliferation and differentiation in the interaction with the inorganic active elements scaffold material, reflecting the promotion of cells proliferation, differentiation and wound healing by using scaffold material.1. It is a simple and quick method to select the higher proliferation and differentiation activity of epidermal stem cells by using typeâ…£collagen.2. Alkaline PH decreased in 24h after culturing, and did not prevent cells from attachment and proliferation. Cell adhesion rate was increased by the presence of inorganic active elements scaffold material. Cells have the best living environment in PH neutral. The inorganic active elements scaffold material created a suitable environment for the proliferation of cells, and their PH attached the B group(only cells).3. The cell proliferation rate in A group was obviously higher than B groups(12d,20d), which indicated that the existence of the inorganic active elements scaffold material could stimulate proliferation and differentiation of cells.4. Inorganic active elements scaffold material can promote the formation of granulation tissue and early healing of the wounds.
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