Preliminary Study On Function Of Long Noncoding RNA MEG3 In HCC And Its Molecular Mechanisms

Objective:Study of human long noncoding RNA maternal imprinted genes 3(MEG3) on cell cycle and apoptosis, and exploring its molecular mechanisms. Methods:Comparison of the high-throughput sequencing of hepatocellular carcinoma and liver tissues, screen out several long noncoding RNAs. Constructed expression vector of these lncRNAs, and selected stable lines with high expression, using qRT-PCR,western blot,RNA-pulldown and microarray techniques to explore the interacting proteins with MEG3 and its mechanisms. Results: By using qRT-PCR to validate screened several lncRNAs in 23 groups of HCC and its adjacent tissues, we found that the expression of MEG3 decreases in most HCC tissues. Finally constructed the eukaryotic expression plasmid of several lncRNAs, screened stable cell lines with high expression of MEG3, acquired several genes which may be relavent to inhibition of cell prolifiration of MEG3 using bioinformation to analyse the microarray. Conclusions: The expression of MEG3 decreases in most HCC tissues; MEG3 could significantly inhibit cell prolifiration, without affect cell cycle and apoptosis, we also acquired stable hepatoma cells strains with high expression of MEG3, and bioinformatic analysis of their expression profiles, selected genes related to MEG3.Combination of literature and preliminary validation, the following factors may be target genes which were regulated by MEG3,such as IL-8,IL-24,IL-1A,CPEB4,CHAC1,BACT1 and so on. These proteins are associated with cell growth and proliferation.