Expression And Clinical Significance Of HMGA1 And HMGA2 In Rhabdomyosarcoma

Purpose To investigate the expression of HMGA1 and HMGA2 in rhabdomyosarcoma,and to evaluate their relationship with clinicopathologic features and cell proliferation index.Methods Immunohistochemical method was adopted to detect the expression of HMGA1,HMGA2 and Ki-67 proteins in 39 cases of RMS and 10 cases of normal skeletal muscle. In situ hybridization was adopted to detect the expression of HMGA1 and HMGA2 on the mRNA level in 39 cases of RMS.Results The positive expression of HMGA1 and HNGA2 were 76.9%(30/39) and 64.1%(25/39) respectively which is higher than that of normal skeletal muscles (P<0.05).The positive expression of HMGA1 and HMGA2 on the mRNA level were 69.2%(27/39)和66.7%(26/39) respectively which is higher than that of normal skeletal muscles (P<0.05). The expressions of HMGA1 and HMGA2 on the protein level were in coincidence with that on the mRNA level. No statistical differences were observed for HMGA1 and HMGA2 expression levels in ERMS and ARMS (P>0.05,respectively), but statistical differences were observed for HMGA1 and HMGA2 expression levels in different differentiated RMS (P<0.05,respectively). The expression of HMGA1 and HMGA2 in RMS with relapse or metastasis were higher than that in RMS without relapse or metastasis, (P<0.05,respectively). Also no statistical differences were observed for both HMGA1 and HMGA2 between Actively-proliferating group(Ki-67 positive) and inactively-proliferating group(Ki-67 negative),(P>0.05, respectively).Conclusions The over expression of HMGA1 and HMGA2 may be significantly involved in the carcinogenesis and progression of RMS and hopefully both of them will be one of prognostic indicators of the tumor.They will also provide a new basis for targeted therapy.