Metabonomics Study On The Biochemical Profiles Of Diabetic Nephropathy

With the improvement of people's living standards, changes of lifestyle, and longer of life expectancy, more and more people suffer from type 2 diabetes mellitus (T2DM) all over the world. Diabetic nephropathy (DN) as a common and severe chronic complication of T2DM has rapidly become one of important public health problem.Here, T2DM and DN metabonomics were investigated, by comparing small molecules in urine samples gotten from T2DM and DN patients and healthy controls, the potential biomarkers of DN were founded. The thesis included three parts:1. A novel method was proposed to simultaneous determine 3 kinds of common used drugs for diabetes in urine samples based on RP-HPLC.2. The ratio of urine protein (PRO) and urine creatinine (UCr) in urine samples of T2DM patient group (without nephropathy period), DN group patients and control group were investigated. The results showed that the increase in urinary PRO/UCr indicates the early renal damage in diabetes patients and it has great significance in diagnosis of T2DM and early DN.3. The morning urine samples were collected from three groups (normal, T2DM and DN patients). And then small molecules in urine samples were investigated using GC/MS. Three different standards were used to normalized GC/MS data including urine volume, creatinine concentration and urine protein content. By t-test results, one compound was identified from significant differences between urine of T2DM and DN patients as potential DN biomarker using urine volume as normalized standard, and four compounds were identified using urine protein as normalized standard.