Analysis Of The Drugs And Endogenous Substances In Biological Samples By Chromatography Coupled To Mass Spectrometry

The combination of chromatography with mass spectrometry, which is based on high separation ability of chromatography together with high sensitivity and selectivity of mass spectrometry, has grown to become a powerful technology in analytical chemistry. Because the online coupling technology can provide lower limit of detection, wider analytical range and better separation ability, it has become such an important analytical technology of separation and identification of structure that it was applied to many fields, such as nature product component analysis, residues analysis, clinical diagnosis and so on.This thesis investigated the following aspects:1,A rapid, sensitive and robust CE/MS method was developed to quantify three tetracyclines in milk. By using online concentration method of field-amplified sample stacking with electromigration injection, the sensitivity was increased for six to seven times. The method has been successfully used to analyze tetracycline residues in bovine milk sold in supermarket.2,A CE/MS method for the simultaneous quantification of nineteen amino acids in human urine was established. The validated method was successfully applied to the determination of amino acids in urine of bladder cancer patient. This research provided investigation information to pathogenesis of bladder cancer.3,An approach for the determination of nucleoside in human urine by HPLC/MS was also established, by using full scan mode, seventy-one endogenous metabolites were detected. The method was applied to investigate urine of bladder cancer patient, and then the statistical analysis was carried out and possible candidate biomarkers of bladder cancer were found.