The Effect Of Quercetin On Mice Fibrob-Last Cell Line L929 Proliferation Through Activating MTOR Signal Pathway

Wound healing, including three successive but overlapping phases, namely inflammatory phase, granulation tissue formation and scar formation. Wound healing mechanism is not fully clear at present that the local wound fibroblasts and the reproduction rate of epidermal cell division to slow down and extracellular matrix synthesis to reduce the decomposition speed is the pathological basis of prolonged unhealed wound. Epidermal growth factor, platelet-derived growth factor (PDGF), TGF-β, bFGF and VEGF promote wound healing and othe cell growth factor and E-selectin, intercellular adhesion molecule-1, vascular cell adhesion and other adhesion molecule-1 reduce the synthesis and secretion of molecules. A variety of metalloproteinases, including collagenase synthesis and secretion lead to pathological changes of chronic refractory wound molecular biology. If can effectively induce the fibroblasts, epidermal cell proliferation of the local wound and increase secretion function and synthesize and secrete of healing of the wound healing factor, you can achieve the purpose of the treatment of chronic wound healing.Objective: This article took mouce fibctrob-last cell line L929 as the research object, exploring the effect of quercetin on mouce fibrob-last cell line L929 proliferation and it's molecular mechanism.Methods: The effect of mouce fibctrob-last cell line L929 proliferation was measured by MTT assay; the effect of quercetin on protein regulation was detected by luciferase reporter assay; the effect of quercetin on the expressions of mTOR, cyclin D1 and p-4E-BP1, key genes in the mTOR-mediated protein translation pathway, was determined by Western Blot analysis.Results: Among the high concentration groups (1, 1.5 ,2 ,2.5×l0^-4M/L )at the point of 36 hours, quercetin significantly induced proliferation of mouce fibrob-last cell line L929; the Luciferase reporter gene detection system showed as follows: Compared with the control group, the RLU (relative light units) of 2.5×l0^-4M/L quercetin value increased significantly at the point of 36 hours; Quercetin increased the expression of mTOR and cyclinD1, and the phosphorylation of 4E-BP1 in mouce fibrob-last cell line L929 by Western blot, and mTOR pathway blocking experiments. After 100ng/ml rapamycin for 36h, compared with the control group, the p-4E-BP1, mTOR and cyclin D1 protein expressions of quercetin group were significantly increased; those of rapamycin group were significantly reduced; and those of rapamycin + quercetin group were increased, but decreased compared with quercetin group.Conclusions: Quercetin can induce mouce fibrob-last cell line L929 proliferation through activating mTOR signal pathway to increase expression of cyclin D1.