Effect Of Baicalin On Proliferation Of Endogenous Neural Stem Cells In Global Cerebral Ischemia/Reperfusion Rats

Objective:To investigate the effect of baicalin in global cerebral ischemia/reperfusion rats,and the probable mechanism involved.Methods:75 SD rats were randomly divided into 3 groups: (1) Sham operation group (S group) 17, only expose three arterie;(2)Ischemia reperfusion group(I/R group)29,bloodletting from the Jugular vein,and transient occlusion of the bilateral common carotid arteries for 20 min;(3)Global cerebral ischemia/reperfusion + baicalin treatment group (I /RB group) 29 ,the same techniques as I/R group.After awaking and 12h after reperfusion , the rats were given baicalin and saline solution by intragastric administration. From the next day after reperfusion, the rats were given baicalin and saline solution by intragastric administration 2 times one day(at9:00 and 15:00 respectively ).S group and I/R group were given the corresponding dose of saline infusion at the same time. Morris water maze test was executed to detect spatial learning and memory. HE staining was performed to observe the the hippocampal pyramidal cell morphology changes. TUNEL staining was carried out to detect the apoptosis of neurons. Immunohistochemistry was used to detect the proliferation cell marker expression of BrdU-positive cells. Western blotting was implemented to detect the expression of COX-2 in hippocampus.Results:Baicalin improved spatial learning and memory damage in global cerebral ischemia/reperfusion rats, attenuated hippocampal pyramidal cell nucleus shrinkage and impairment of pyramidal cells, reduced number of apoptotic neurons in the hippocampus CA1 area, increased number of proliferous neural stem cells in global cerebral ischemia/reperfusion rats, and reduced the expression of COX-2 in hippocampus.Conclusions:Baicalin can improve the cognitive dysfunction in global cerebral ischemia/reperfusion rats and ameliorate their spatial memory, reduce brain damage, decrease number of apoptotic neurons in the hippocampus CA1 area, and motivate the proliferation of endogenous neural stem cells in global cerebral ischemia/reperfusion rats, which may be through inhibiting cerebral ischemia / reperfusion brain inflammation, thereby improving the neural stem cell niche (niche), coming up to promote endogenous neural stem cells proliferation.